| CatalogCode: | NB100-289 |
| ProductName: | MCM3 Antibody |
| Product Description: | Rabbit Polyclonal anti-MCM3 |
| Clonality: | Polyclonal |
| Immunogen: | A 38-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 10 and 11 (LocusLink ID 4172). |
| Specificity: | This antibody is specific for human MCM3. |
| CrossReactivity: | NB 100-289 recognizes human and Xenopus MCM3. Other species have not been tested. Reactivity with mouse is likely based upon sequence identity (31/38 identical residues). |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | Western blot and immunoprecipitation.Suggested working dilutions: Western Blot - 1: 2,000-1:10,000 Immunohistochemistry/Immunocytochemistry - NDImmunoprecipitation - 2 to 5 ug/mg lysate***The investigator should determine the optimal working dilution for a specific application.**Use NB 100-288 (MCM2 antibody) to IP the MCM complex |
| Background: | MCM3 is one of the highly conserved mini-chromosome maintenance proteins that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Host_Name: | Rabbit |
| Buffer: | Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide |
| ListPrice: | 285 |
| AppSummary: | WB, IP |
| SpeciesSummary: | Hu |
| ALTnames: | anti-RLFB antibody, anti-replication licensing factor antibody, anti-beta subunit antibody, anti-P1-MCM3 antibody, anti-P1 Protein antibody, anti-DNA polymerase alpha holoenzyme-associated protein P1 antibody, anti-P102 protein antibody, anti-HCC5 antibody, anti-human cervical cancer proto-oncogene 5 antibody |
| ProteinTarget: | MCM3 |
| PackageSize: | 0.1 ml |
| NotesMain: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |
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