| CatalogCode: | NB100-2840 |
| ProductName: | Ku80 Antibody |
| Product Description: | Goat Polyclonal anti-Ku80 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: C-SLAKKDEKTDTLED, representing the internal region of the human protein according to NP_066964.1 |
| CrossReactivity: | Reactivity based upon sequence homology: Human |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 80-85kDa band observed in lysates of HeLa (calculated MW of 82.7kDa according to NP_066964.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of 75kDa was observed consistent with products from other sources. |
| Background: | Eukaryotic cells contain an abundant nuclear protein, Ku, which was initially discovered as an autoantigen. Ku is a heterodimer of 70 and 80 kDa subunits which binds to DNA ends and other alterations in double-stranded DNA including hairpins, nicks and gaps. Ku plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). Ku targets DNA-PK to DNA. The Ku/DNA-PK complex might provide a structural framework for holding broken chromosomes together during repair. Ku plays a role in many diverse processes including cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis (2-4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Host_Name: | Goat |
| Buffer: | Tris-saline containing 0.02% Na azide [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-ATP dependent DNA helicase II 80 kDa subunit antibody, anti-ATP dependent DNA helicase II 86 Kd subunit antibody, anti-CTC box binding factor 85 kDa antibody, anti-CTC85 antibody, anti-CTCBF antibody, anti-DNA repair protein XRCC5 antibody, anti-KARP1 antibody, anti-Ku86 autoantigen related protein 1 antibody, anti-NFIV antibody, anti-Nuclear factor IV antibody, anti-Thyroid lupus autoantigen antibody, anti-TLAA antibody, anti-Xray repair complementing defective repair in Chinese hamster cells 5 antibody |
| ProteinTarget: | XRCC5 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Mayeur GL, Kung WJ, Martinez A, Izumiya C, Chen DJ, Kung HJ. Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells. J Biol Chem. 2005 Mar 18;280(11):10827-33. Epub 2005 Jan 7. PMID: 15640154 |
| Gene_Id: | 7520 |
| Omim_ID: | NB100-337 |
|