| CatalogCode: | NB100-2620 |
| ProductName: | APC Antibody |
| Product Description: | Mouse Monoclonal anti-APC (c-APC 28.9) |
| Clone: | c-APC 28.9 |
| Clonality: | Monoclonal |
| Immunogen: | human APC C-terminal fusion with MBP (maltose binding protein) |
| Specificity: | This antibody has been used routinely for Western blotting on colon cell line HCT116 extract which expresses full-length APC. |
| CrossReactivity: | Cross-reacts with Human and Mouse.Not yet tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | IF: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 300 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | colon cell line HCT116 |
| Background: | Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline |
| ListPrice: | 295 |
| AppSummary: | IF, IP, WB, IHC-Fr |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-Adenomatous Polyposis Coli antibody, anti-APC antibody, anti-DP2 antibody, anti-DP2.5 antibody, anti-DP3 antibody, anti-FAP antibody, anti-FPC antibody, anti-GS antibody |
| ProteinTarget: | APC |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Fodde R et al. Mutations in the APC tumour suppressor gene cause chromosomal instability. Nat Cell Biol 3:433-8 (2001). Efstathiou JA et al. Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells. Proc Natl Acad Sci U S A 95:3122-7 (1998). |
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