| CatalogCode: | NB100-2488 |
| ProductName: | IL13 Antibody |
| Product Description: | Mouse Monoclonal anti-IL13 (32116.11) |
| Clone: | 32116.11 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length human IL13 protein expressed in E. coli. |
| Specificity: | Recognizes recombinant human IL-13 by capture ELISA and neutralization. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg Protein A purified Mouse ascites. |
| Uses: | ELISA: Use in combination with a biotinylated IL13 polyclonal detection antibody at 400 ng/ml (100 ul/well), an ELISA for sample volumes of 100 ul with a range of 62.5-4000 ug/ml can be obtained. Neut: Use at a concentration of 1-3 ug/ml in the presence of 10 ng/ml of recombinant human IL13 using the human erythroleukemia cell line, TF-1. WB: Detects a band of approximately 9 kDa (predicted molecular weight: 12 kDa). The detection limit for recombinant human IL13 is approximately 50 ng/lane under non-reducing and reducing conditions. Endotoxin level is < 10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Secreted |
| Control: | ab10782 has the ability to neutralize the bioactivity of human IL13 in the human erythroleukemia cel |
| Background: | Human IL-13 was originally identified by differential screening of an anti-CD28 activated human peripheral blood mononuclear cell cDNA library as an induction-specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL-13 (P600) cDNA as a probe.2 Human IL-13, a pleiotropic cytokine, is produced by activated Th0, Th1-like, Th2-like, and CD8 T cells.1 The gene for human IL-13 maps to chromosome 5 and is closely linked to the genes for IL-3, IL-4, IL-5, and GM-CSF.3 IL-13 inhibits proinflammatory cytokine production and stimulates antibody production.1 It induces proliferation in the human pre-myeloid cell line TF-1. IL-13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression.4 IL-13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity.5 |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | ELISA, WB, CA |
| SpeciesSummary: | Hu |
| ALTnames: | anti-ALRH antibody; anti-IL-13 antibody; anti-MGC116786 antibody; anti-MGC116788 antibody; anti-MGC116789 antibody; anti-P600 antibody |
| ProteinTarget: | IL13 |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:McKenzie AN et al. Structural comparison and chromosomal localization of the human and mouse IL-13 genes. J Immunol 150:5436-44 (1993). McKenzie AN et al. Interleukin 13, a T-cell-derived cytokine that regulates human monocyte and B-cell function. Proc Natl Acad Sci U S A 90:3735-9 (1993). Doherty TM et al. Modulation of murine macrophage function by IL-13. J Immunol 151:7151-60 (1993). Cherwinski HM et al. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med 166:1229-44 (1987). |
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