| CatalogCode: | NB100-2414 |
| ProductName: | NOTCH3 Antibody |
| Product Description: | Goat Polyclonal anti-NOTCH3 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: C-QLGPQPEVTPKRQ, representing the C Terminus of the human protein according to NP_000426.1 |
| CrossReactivity: | Expected reactivity based upon sequence homology: Human, Mouse, Rat |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:16,000.
Western Blot: Preliminary experiments gave approx 60+70kDa bands in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Background: | Gene ID(s): 4854 (human) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline containing 0.02% Na azide [pH7.3] with 0.5% BSA |
| ListPrice: | 195 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-NOTCH3 antibody, anti-Notch homolog 3 (Drosophila) antibody, anti-HGNC:7883 antibody, anti-CADASIL antibody, anti-CASILantibody, anti-Notch (Drosophila) homolog 3 antibody, anti-Notch homolog 3 antibody |
| ProteinTarget: | NOTCH3 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Dang L, Fan X, Chaudhry A, Wang M, Gaiano N, Eberhart CG. Notch3 signaling initiates choroid plexus tumor formation. Oncogene. 2005 Sep 26; [Epub ahead of print] PMID: 16186803
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| NotesMain: | **Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). |
| Gene_Id: | 4854 |
| Omim_ID: | H00004854-A01 |
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