| CatalogCode: | NB100-2404 |
| ProductName: | Eotaxin Antibody |
| Product Description: | Rat Monoclonal anti-Eotaxin (42285.111) |
| Clone: | 42285.11 |
| Clonality: | Monoclonal |
| Immunogen: | Purified recombinant mouse eotaxin expressed in E. coli. |
| Specificity: | Recognizes recombinant mouse eotaxin. From ELISA and WB, this antibody exhibits less than 10 % cross-reactivity with recombinant human eotaxin, recombinant mouse MARC, and recombinant human 6Ckine. |
| CrossReactivity: | Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 mg Rat ascites. |
| Uses: | In Western Blot an 8.4 kDa band is seen.ELISA: Detection Limit: 1.2 ng/well*Optimal dilutions should be determined by the end user. |
| Localization: | Secreted |
| Background: | Eotaxin, a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines, was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Eotaxin has been found in three species (human, mouse, and guinea pig), which share approximately 60 % sequence homology.1 Mouse eotaxin also shows high sequence identity to the MCP family of cytokines. Mouse eotaxin cDNA 2 encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse eotaxin. Mature mouse eotaxin has a predicted molecular mass of approximately 8.4 kDa. Eotaxin is a potent eosinophil activator and chemoattractor. As an activator, eotaxin is a mediator of inflammation by initiating actin polymerization intracellularly and inducing the production of reactive oxygen species.3 As a chemoattractant both in vivo and in vitro, eotaxin exhibits potent activity on eosinophils, but not mononuclear cells or neutrophils.3, 4, 5 Eotaxin has the ability to prime eosinophils for chemotaxis, direct their migration, and to activate inflammatory activity. Eotaxin mRNA is expressed in a variety of tissues. Its expression is induced in cultured endothelial cells in response to IFN- |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG2a |
| Host_Name: | Rat |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | ELISA, WB, CA |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-CCL11 antibody; anti-Chemokine CC Motif Ligand 11 antibody; anti-Eosinophil Chemotactic Protein antibody; anti-MGC22554 antibody; anti-SCYA11 antibody; anti-Small inducible cytokine subfamily A member 11 antibody |
| ProteinTarget: | Eotaxin |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Kitaura, M., et al., Molecular cloning of humaneotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J. Biol. Chem., 271, 7725-7730 (1996). 2. Rothenberg, M.E., et al., Murine eotaxin: an eosinophil chemoattractant inducible in endothelial cells and in interleukin 4-induced tumor suppression. Proc. Natl. Acad. Sci. USA, 92, 8960-8964 (1995). 3. Elsner, J., et al., Human eotaxin represents a potent activator of the respiratory burst of human eosinophils. Eur. J. Immunol., 26, 1919-1925 (1996). 4. Griffiths-Johnson, D.A., et al., The chemokine, eotaxin, activates guinea-pig eosinophils in vitro and causes their accumulation into the lung in vivo. Biochem. Biophys. Res. Commun., 197, 1167-1172 (1993). 5. Garcia-Zepeda, E.A., et al., Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nat. Med., 2, 449-456 (1996). 6. Gao, J.L., et al., Identification of a mouse eosinophil receptor for the CC chemokine eotaxin. Biochem. Biophys. Res. Commun., 223, 679 (1996). |
| NotesMain: | Endotoxin: <10 ng/mg antibody determined by the LAL method. |
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