| CatalogCode: | NB100-2381 |
| ProductName: | VEGFA Antibody |
| Product Description: | Rabbit Polyclonal anti-VEGFA |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide made to an internal region of the human VEGFA protein sequence (between residues 150-250). [Swiss-Prot# P15692] |
| Specificity: | This antibody is specific for VEGFA. |
| CrossReactivity: | This antibody reacts with human protein. Other species have not yet been tested. The immunogen used to produce this antibody has 100% homology with mouse, rat, pig, hamster, horse, chicken, guinea pig, dog, and cow proteins. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody is useful for Western blot analysis, where a band is seen at ~42 kDa with a chimera transfected lysate, representing the homodimer VEGFA. Another faint band is seen at ~21 kDa, representing the monodomer form of VEGFA.Suggested Starting Dilution:*Western Blot: 1 ug/ml (ECL)*Optimal working dilutions should be determined by the investigator. |
| Localization: | Secreted |
| Background: | VEGFA is a member of the PDGF/VEGF growth factor family and is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| Buffer: | Tris-citrate/phosphate buffer, pH 7-8 |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Vascular endothelial growth factor A antibody; anti-Vascular endothelial growth factor A precursor antibody; anti-Vascular permeability factor antibody; anti-VPF antibody |
| ProteinTarget: | VEGFA |
| PackageSize: | 0.1 ml |