| CatalogCode: | NB100-2249 |
| ProductName: | eIF2-alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-eIF2-alpha |
| Clonality: | Polyclonal |
| Immunogen: | The epitope recognized by NB 100-2249 maps to a region between residue 275 and the C-terminus (residue 315) of human Eukarytic Translation Initiation Factor 1, subunit 1 alpha, 35kDa using the numbering given in entry NP_004085.1 (GeneID 1965). |
| CrossReactivity: | Human and mouse. Other species have not been tested. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.Suggested starting dilutions are as follows:*Western Blot: 1:2,000 to 1:10,000Immunoprecipitation: 2 to 5 mcg/mg lysateImmunohistochemistry: not testedImmunocytochemistry: not tested*Investigator should determine optimal working conditions and dilutions. |
| Background: | eIF-2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF-2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF-2 alpha is phosphorylated at serine 52 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF-2 alpha blocks the GDP-GTP exchange activity of eIF-2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF-2 alpha is an important regulatory process in protein synthesis. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| Buffer: | Tris-buffered Saline containing 0.1% BSA |
| ListPrice: | 295 |
| AppSummary: | WB, IP |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-eIF2a antibody, anti-eIF-2A antibody, anti-eIF2-alpha antibody, anti-eIF-2alpha antibody, anti-Eukaryotic Translation Intitiation Factor 2A antibody, anti-EIF2S1 antibody, anti-Eukaryotic Translation Initiation Factor 2 antibody, anti-subunit 1 alpha antibody, anti-35kDa antibody |
| ProteinTarget: | eIF2-alpha |
| PackageSize: | 0.1 ml |
| Gene_Id: | 1965 |
| Omim_ID: | NB120-4775 |
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