| Catalog Code: | NB100-2238 |
| Product Type: | Primary Antibody |
| Product Name: | Nucleolin Antibody |
| List Price: | 295 |
| Clonality: | Polyclonal |
| Gene ID: | 4691 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-Nucleolin - Nucleolus Marker |
| Cross Reactivity: | Human. Initial blots performed on lysate from NIH3T3 cells failed to provide evidence that this antibody reacts with the mouse ortholog. Other species have not been tested. |
| Species Summary: | Hu |
| Background: | Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. I |
| Alternate Names: | anti-nucl antibody, anti-protein C23 antibody, anti-NCL antibody, anti-Nucleolin antibody, anti-C23 antibody |
| Immunogen: | The epitope recognized by NB 100-2238 maps to a region between residue 525 and 575 of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691). |
| Purity: | Affinity purified |
| Buffer: | Tris-buffered Saline containing 0.1% BSA |
| Packaging: | 0.1 ml of affinity purified rabbit antisera. |
| Package Size: | 0.1 ml |
| Concentration: | 0.2 mg/ml |
| Uses: | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows:* Western Blot: 1:2,000 to 1:10,000 Immunoprecipitation: 2 to 5 mcg/mg lysate I |
| Application Summary: | WB, IP |