| CatalogCode: | NB100-2237 |
| ProductName: | Nucleolin Antibody |
| Product Description: | Rabbit Polyclonal anti-Nucleolin - Nucleolus Marker |
| Clonality: | Polyclonal |
| Immunogen: | The epitope recognized by NB 100-2237 maps to a region between residue 250 and 300 of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691). |
| CrossReactivity: | Human. Initial blots performed on lysate from NIH3T3 cells failed to provide evidence that this antibody reacts with the mouse ortholog. Other species have not been tested. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.Suggested starting dilutions are as follows:*Western Blot: 1:2,000 to 1:10,000Immunoprecipitation: 2 to 5 mcg/mg lysateImmunohistochemistry: not testedImmunocytochemistry: not tested*Investigator should determine optimal working conditions and dilutions. |
| Background: | Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. It is also a good mammalian nucleolus marker. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| Buffer: | Tris-buffered Saline containing 0.1% BSA |
| ListPrice: | 295 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-nucl antibody, anti-protein C23 antibody, anti-NCL antibody, anti-Nucleolin antibody, anti-C23 antibody |
| ProteinTarget: | Nucleolin (NCL) |
| PackageSize: | 0.1 ml |
| Marker: | Nucleolus Marker |
| Gene_Id: | 4691 |
| Omim_ID: | NB100-2238 |
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