| CatalogCode: | NB100-2177 |
| ProductName: | BCRP Antibody |
| Product Description: | Mouse Monoclonal anti-BCRP (BXP-21) |
| Clone: | BXP-21 |
| Clonality: | Monoclonal |
| Immunogen: | Fusion protein composed of E. Coli maltose binding protein and BCRP peptide [126 amino acids, 271-396 AA of BCRP. |
| Specificity: | This antibody recognizes an internal epitope of human BCRP (70 kDa), a transmembrane half-transporter. The antibody does not cross-react with the human MDR1, MRP1, and MRP2 gene products. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.25 ml Tissue culture supernatant Mouse ascites. |
| Uses: | ICC: Using immunocytochemistry on acetone-fixed, frozen cytospin preparations.IHC: Using immunohistochemistry on acetone-fixed, frozen sections usingrabbit anti-mouse IgG and a APAAP complex. The antibody can be used on formalin-fixed, paraffinembedded human tissues and tumors after pretreatment with 10 mM citric acid, pH 6.0, for 20 minutes.WB: Use at assay dependant dilution. |
| Localization: | Plasma membrane; integral membrane protein |
| Background: | Tumor cells may display a multidrug resistant phenotype by overexpression of ATP-binding cassette transporters such as multidrug resistance (MDRI) p-glycoprotein, multidrug resistance protein 1 (MRP1), and breast cancer resistance protein (BCRP). Tumor cells can be intrinsically resistant to drugs or they can acquire resistance to structurally and functionally unrelated drugs on drug exposure. This phenomenon is known as multidrug resistance (MDR). In human tumor cells, several transporter proteins can be involved in MDR. These proteins, MDR1 P-gp (ABCB1), MRP1 (ABCC1), MRP2 (ABCC2), MRP3 (ABCC3), and BCRP (ABCG2), belong to the ABC transporter family. They act as efflux pumps, which result in decreased intracellular concentrations of cytotoxic drugs. BCRP is a recently discovered half-transporter that probably acts as a homo- or heterodimer in transporting cytotoxic agents. The transporter molecule is capable of transporting several anticancer drugs but has thus far been found mainly in MX-resistant cell lines. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | PBS with 0.7% BSA |
| ListPrice: | 295 |
| NovusRef: | 1. Ejendal, K., et al. The nature of amino acid 482 of human ABCG2 affects substrate transport and ATP hydrolysis but not substrate binding. Protein Sci., 15: 1597-1607, Jul 2006. |
| AppSummary: | WB, ICC, IHC-P, IHC-Fr |
| SpeciesSummary: | Hu |
| ALTnames: | Anti-ABC15 antibody; Anti-ABCG 2 antibody; Anti-ABCG2 antibody; Anti-ABCP antibody; Anti-ATP-binding cassette sub-family G member 2 antibody; Anti-BCRP1 antibody; Anti-BMDP antibody; Anti-Breast cancer resistance protein antibody; Anti-CDw338 antibody; Anti-CDw338 antigen antibody; Anti-EST157481 antibody; Anti-MGC102821 antibody; Anti-Mitoxantrone resistance associated protein antibody; Anti-MRX antibody; Anti-MXR antibody; Anti-MXR1 antibody; Anti-Placenta-specific ATP-binding cassette transporter antibody |
| ProteinTarget: | BCRP / ABCG2 [BXP-21] |
| PackageSize: | 0.25 ml |
| GeneralRef: | 1. Doyle, L.A., et al., A multidrug resistance transporter from human MCF-7 breast cancer cells. Proc. Natl. Acad. Sci. USA, 95, 15665-15670 (1998). 2. Scheffer, G.L., et al., Breast Cancer Resistance Protein is localized at the plasma membrane in mitoxantrone and topotecan resistant cell lines. Cancer Res., 60, 2589-2593 (2000). 3. Maliepaard, M., et al., Subcellular localization and distribution of the breast cancer resistance protein transporter in normal human tissues. Cancer Res., 61, 3458-3464 (2001). |
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