| CatalogCode: | | NB100-2176 |
| ProductName: | | NF-kB Antibody |
| Product Description: | | Rabbit Polyclonal anti-NF-kB |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide corresponding to the C-terminus of human NFkB p65. |
| Specificity: | | Reacts with NFkB p65 |
| CrossReactivity: | | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | | 1 ml IgG purified Rabbit antisera. |
| Uses: | | In Western Blot this antibody detects a band of approximately 64 kDa (predicted molecular weight: 69 kDa). |
| Localization: | | Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B). |
| Control: | | K-562, NIH/3T3 and A-431 |
| Background: | | p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer. |
| ProductRef: | | 1. Starkey, J. M., Haidacher, S. J., LeJeune, W. S., Zhang, X., Tieu, B. C., Choudhary, S., Brasier, A. R., Denner, L. A., and Tilton, R. G. (2006) Diabetes-Induced Activation of Canonical and Noncanonical Nuclear Factor-{kappa}B Pathways in Renal Cortex, 55, 1252-1259. |
| Storage: | | Store at 4C. Do not freeze. |
| Purity: | | IgG purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | PBS with 0.2 gelatin |
| ListPrice: | | 325 |
| AppSummary: | | IHC, IP, WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-NFKB 3 antibody, anti-NFKB3 antibody, anti-Nuclear Factor NF Kappa B p65 Subunit antibody, anti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody, anti-RELA antibody, anti-Transcription Factor p65 antibody, anti-V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody |
| ProteinTarget: | | NF-kB (p65) |
| PackageSize: | | 1 ml |
| GeneralRef: | | 1. Lentsch AB, Ward PA. The NFkappaBb/IkappaB system in acute inflammation. Arch Immunol Ther Exp (Warsz). 48(2): 59-63. 2. Hatada EN, Krappmann D, Scheidereit C. NF-kappaB and the innate immune response. Curr Opin Immunol. 2000 Feb; 12(1): 52-8 3. Ho E, Bray TM. Antioxidants, NFkappaB activation, and diabetogenesis. Proc Soc Exp Biol Med. 1999 Dec; 222(3): 205-13. 4. Valen G, Yan ZQ, Hansson GK. Nuclear factor kappa-B and the heart. J Am Coll Cardiol. 2001 Aug; 38(2): 307-14. 5. Chen FE, Kempiak S, Huang DB, Phelps C, Ghosh G. Construction, expression, purification and functional analysis of recombinant NFkappaB p50/p65 heterodimer. Protein Eng. 1999 May; 12(5): 423-8. 6. Sengchanthalangsy LL, Datta S, Huang DB, Anderson E, Braswell EH, Ghosh G. Characterization of the dimer interface of transcription factor NFkappaB p50 homodimer. J Mol Biol. 1999 Jun 18; 289(4): 1029-40. 7. Huang DB, Huxford T, Chen YQ, Ghosh G. The role of DNA in the mechanism of NFkappaB dimer formation: crystal structures of the dimerization domains of the p50 and p65 subunits. Structure. 1997 Nov 15; 5(11): 1427-36. 8. Magnani M, Crinelli R, Bianchi M, Antonelli A. The ubiquitin-dependent proteolytic system and other potential targets for the modulation of nuclear factor-kB (NF-kB). Curr Drug Targets. 2000 Dec; 1(4): 387-99. |
| more information: | | company product webpage |
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