| CatalogCode: | | NB100-2077 |
| ProductName: | | DNA PKcs Antibody |
| Product Description: | | Mouse Monoclonal anti-DNA PKcs (10B1) [Thr2609] |
| Clone: | | 10B1 |
| Clonality: | | Monoclonal |
| Immunogen: | | Synthetic modified peptide. |
| CrossReactivity: | | Cross-reacts with Human Thr2609-phosphorylated DBA PKcs. Not yet tested in other species. |
| Packaging: | | 0.05 mg protein G purified Mouse ascites. |
| Uses: | | This antibody can be used in Immunofluorescence and Western Blot. In Western Blot a 40kDa band is seen.*Optimal dilutions should be determined by the end user. |
| Localization: | | Nuclear |
| Control: | | MOLT4 nuclear extract treated with 2 to 10 Gy radiation. |
| Background: | | DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damage. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | protein G purified |
| Isotype: | | IgG1 kappa |
| Host_Name: | | Mouse |
| Buffer: | | PBS [pH 7.2] |
| ListPrice: | | 295 |
| AppSummary: | | IF, WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-DNAPK catalytic subunit antibody, anti-DNPK1 antibody, anti-HYRC1 antibody, anti-p460 antibody, anti-PKRDC antibody, anti-Protein Kinase DNA Activated Catalytic Polypeptide antibody, anti-XRCC7 antibody |
| ProteinTarget: | | DNA PKcs (phospho T2609) |
| PackageSize: | | 0.05 mg |
| GeneralRef: | | 1. Hartley, K., et al., 1995. Cell. 82:849.2. Connelly, M., et al., 1996. Gene. 175:271.3. Douglas, P., et al., 2002. Biochem. J. 368:2434. Calsou, P., et al., 2003. J. Mol. Biol. 326:93. |
| Phosphorylated: | | Thr2609 |
| more information: | | company product webpage |
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