| CatalogCode: | NB100-2059 |
| ProductName: | Thrombospondin Antibody |
| Product Description: | Mouse Monoclonal anti-Thrombospondin (A6.1) |
| Clone: | A6.1 |
| Clonality: | Monoclonal |
| Immunogen: | BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. |
| Specificity: | This antibody is specific to 450 kD protein, a non-reduced form, and 170 to 180 kD protein, a reduced form, of Thrombospondin. |
| CrossReactivity: | Human, cow, pig, horse, dog, sheep, mouse and rat. Not tested in other species. |
| Packaging: | 0.25 ml IgG purified Mouse ascites. |
| Uses: | Western Blot: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Localization: | Secretory granules, Golgi complex, endoplasmic reticulum |
| Control: | Tonsil or placenta |
| Background: | Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Antibody solution with sodium azide |
| ListPrice: | 295 |
| AppSummary: | IF, IP, WB, IHC-P |
| SpeciesSummary: | Bv, Ca, Hu, Mu, Po, Rt, Eq, Sh |
| ALTnames: | Anti-THBS 1 antibody; Anti-THBS antibody; Anti-THBS1 antibody; Anti-Thrombospondin 1 antibody; Anti-Tsp 1 antibody; Anti-TSP antibody; Anti-Tsp1 antibody |
| ProteinTarget: | Thrombospondin |
| PackageSize: | 0.25 ml |
| GeneralRef: | i) Dixit et al. Biochemistry 24 (16): 4270, 1985.
ii) Dixit et al. J Bio Chem 261 (4): 1962, 1986.
Iii) Galvin et al. J Cell Bio 104 (5): 1413, 1987 |
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