| CatalogCode: | | NB100-2033 |
| ProductName: | | IkB beta Antibody |
| Product Description: | | Rabbit Polyclonal anti-IkB beta |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide (IkB beta) conjugated to KLH - which corresponds to a region near the C-terminus of the human protein. |
| Specificity: | | Recognition is at the highly conserved C-terminal region. |
| CrossReactivity: | | Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | | 0.1 ml Liquid (sterile filtered) |
| Uses: | | Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 45 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | | Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta. Interestingly, IkB-alpha and IkB-beta mediate different NFkB responses. IkB-alpha appears to control more transient activation of NFkB in response to an inducer, while IkB-beta controls a persistent response. Bcl-3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkB is confirmed by immunoblotting. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Whole antisera |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Preservative: 0.01% Sodium Azide |
| ListPrice: | | 325 |
| AppSummary: | | ELISA, IP, WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ProteinTarget: | | IkB beta |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | General / background references:Baldwin AS The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol 14:649-83 (1996). |
| more information: | | company product webpage |