| CatalogCode: | NB100-1993 |
| ProductName: | Dynamin 1 Antibody |
| Product Description: | Mouse Monoclonal anti-Dynamin 1 (D5) |
| Clone: | D5 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant His-tagged fusion protein, corresponding to amino acids 1-750 of Human Dynamin. |
| Specificity: | Detects a band at approximately 100kDa. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Cow and Chicken. Not yet tested in other species. |
| Packaging: | 0.5 mg |
| Uses: | IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 mg/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Microtubule associated. |
| Control: | Mouse Brain Tissue Extract. |
| Background: | Dynamin is a 100 kDa GTP binding protein that was first identified as a protein able to bind microtubules (1). A mutation in the Drosophila gene shits, encoding a dynamin homologue, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles (2). Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles (3). Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this complex (4). To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific, Dynamin 2 is ubiquitously expressed and Dynamin 3 localizes to testis, brain and lung (5). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Protein G purified |
| Isotype: | IgG |
| Host_Name: | Mouse |
| Buffer: | PBS pH7.2, 0.1mM PMSF and 50% glycerol |
| ListPrice: | 285 |
| AppSummary: | IHC, IP, WB |
| SpeciesSummary: | Hu, Mu, Rt, Bo, Ck |
| ProteinTarget: | Dynamin 1 |
| PackageSize: | 0.05 mg |
| GeneralRef: | 1. Shpetner, H.S. and Vallee, R.B. (1989) Cell 59: 421-432. 2. van der Bliek, A.M. and Meyerowitz, E.M. (1991) Nature 351: 411-414. 3. Takei, K., McPherson, P.S., Schmid, S.L., and Ce Camilli, P. (1995) Nature 374: 186-190. 4. Slepnev, V.I., Ochoa, G.-C., Butler, M.H., Grabs, D. and De Camilli, P. (1998) Science 281: 821-824. 5. Cao, H., Garcia, F., McNiven, M.A. (1998) Mol. Biol. Cell. 9: 2595-2609. |
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