| CatalogCode: | NB100-1985 |
| ProductName: | CD74 Antibody |
| Product Description: | Mouse Monoclonal anti-CD74 (PIN.1) |
| Clone: | PIN.1 |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide: DQKPVMDDQRDLISNNE conjugated to KLH, corresponding to amino acids 12-28 of Human CD74. |
| Epitope: | The epitope is in a region of the cytoplasmic tail of human CD74 which is common to all isoforms. |
| Specificity: | This antibody detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse ascites. |
| Uses: | WB: Predicted molecular weight: 33 kDa. 1 ug/ml of this antibody was sufficient to detect CD74 in 20 ug of human B lymphoblastoid cell lysate. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type II membrane protein |
| Control: | Human B lymphoblastoid cell lysate. |
| Background: | CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41kDa, depending on genetic splicing. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2. |
| ListPrice: | 295 |
| AppSummary: | FACS, IHC, IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-CD 74 antibody, anti-CD74 antigen antibody, anti-CD74 molecule antibody, anti-CLIP antibody, anti-DHLAG antibody, anti-Gamma chain of class II antigens antibody, anti-HLA class II histocompatibility antigen gamma chain antibody, anti-HLA DR antigens associated invariant chain antibody, anti-HLA DR gamma antibody, anti-HLADG antibody, anti-HLADR antigens associated invariant chain antibody, anti-Ia antigen associated invariant chain antibody, anti-Ia associated invariant chain antibody, anti-Ia gamma antibody, anti-Ii antibody, anti-Invariant polypeptide of major histocompatibility complex class II antigen associated antibody, anti-Major histocompatibility complex class II invariant chain antibody, anti-MHC HLA DR gamma chain antibody, anti-p33 antibody, anti-p35 antibody, anti-Protein 41 antibody |
| ProteinTarget: | CD74 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Shi GP et al. Role for cathepsin F in invariant chain processing and major histocompatibility complex class II peptide loading by macrophages. J Exp Med 191:1177-86 (2000). Hammond C et al. The tetraspan protein CD82 is a resident of MHC class II compartments where it associates with HLA-DR, -DM, and -DO molecules. J Immunol 161:3282-91 (1998). Watts C Capture and processing of exogenous antigens for presentation on MHC molecules. Annu Rev Immunol 15:821-50 (1997). |
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