| Catalog Code: | NB100-1979 |
| Product Type: | Primary Antibody |
| Product Name: | Nucleolin Antibody |
| List Price: | 275 |
| Clonality: | Monoclonal |
| Gene ID: | 4691 |
| Host: | Mouse |
| Clone: | 4E2 |
| Isotype: | IgG1 |
| Product Description: | Mouse Monoclonal anti-Nucleolin (4E2) |
| Cross Reactivity: | Cross-reacts with Human and Cow. Does not cross-react with Mouse. Not yet tested in other species. |
| Species Summary: | Hu, Bv, Mu(-) |
| Background: | Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. I |
| Alternate Names: | anti-nucl antibody, anti-protein C23 antibody, anti-NCL antibody, anti-Nucleolin antibody, anti-C23 antibody |
| Immunogen: | Human nucleolin protein from Raji cell extract. |
| Localization: | Nuclear, located mainly in dense fibrillar regions of the nucleolus. |
| Purity: | Protein A purified |
| Buffer: | Preservative: None Constituents: 50% Glycerol, PBS |
| Packaging: | 50 micrograms |
| Package Size: | 0.05 mg |
| Concentration: | 1 mg/ml |
| Uses: | ICC: Use at a concentration of 1 - 10 ug/ml. IHC: Use at a concentration of 1 - 10 ug/ml. WB: Use at a concentration of 1 ug/ml. Predicted molecular weight: 100 kDa. This antibody allows in situ detection of nucleolar protein in Hep-II cell and paraffin e |
| Application Summary: | ICC, IHC, WB |
| General References: | Bharti AK et al. Identification of a nucleolin binding site in human topoisomerase I. J Biol Chem 271:1993-7 (1996). Tuteja N et al. Human DNA helicase IV is nucleolin, an RNA helicase modulated by phosphorylation. Gene 160:143-8 (1995). < |