| CatalogCode: | NB100-1974 |
| ProductName: | Calnexin - ER Marker Antibody |
| Product Description: | Rabbit Polyclonal anti-Calnexin - ER Marker - ER Marker |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: EEDEILNRSPRNRKPRRE conjugated to KLH, corresponding to amino acids 575-593 of Human Calnexin. |
| CrossReactivity: | Cross-reacts with Rat, Cow, Chicken, Drosophilia melanogaster, Guinea pig, Hamster, Monkey, Pig, Quail, Rabbit and Sheep. Expected to cross-react with Human (100% identity with immunogen), Mouse (100% identity with immunogen), Xenopus laevis (100% identity with immunogen) and Dog (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml protein A purified Rabbit antisera. |
| Uses: | WB: this dilution was sufficient for detection of Calnexin in 20 ug of HeLa heat shocked cell lysate. Detects a band of approximately 90 kDa. (predicted molecular weight: 71 kDa). |
| Localization: | Endoplasmic reticulum |
| Control: | Heat Shocked HeLa Cell Lysate. |
| Background: | Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Constituents: Whole Serum |
| ListPrice: | 285 |
| AppSummary: | FACS, IP, WB, ICC |
| SpeciesSummary: | Bv, Ch, Dr, Ha, Mk, Rt, Rb, Gp, Sh |
| ALTnames: | anti-CANX antibody, anti-CNX antibody, anti-FLJ26570 antibody, anti-Histocompatibility complex class I antigen binding protein p88 antibody, anti-IP90 antibody, anti-P90 antibody |
| ProteinTarget: | calnexin |
| PackageSize: | 0.1 ml |
| Marker: | ER Marker |
| GeneralRef: | Hammond C & Helenius A Quality control in the secretory pathway: retention of a misfolded viral membrane glycoprotein involves cycling between the ER, intermediate compartment, and Golgi apparatus. J Cell Biol 126:41-52 (1994). Ou WJ et al. Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature 364:771-6 (1993). Wada I et al. SSR alpha and associated calnexin are major calcium binding proteins of the endoplasmic reticulum membrane. J Biol Chem 266:19599-610 (1991). |
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