| CatalogCode: | NB100-1964 |
| ProductName: | 14-3-3 beta + zeta Antibody |
| Product Description: | Mouse Monoclonal anti-14-3-3 beta + zeta (22-IID8B) |
| Clone: | 22-IID8B |
| Clonality: | Monoclonal |
| Immunogen: | Lysed transformed amnion cells (AMA) (Human). |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Dog, Monkey, Pig, Rabbit and Sheep. Not yet tested in other species. |
| Packaging: | 0.05 mg Mouse ascites. |
| Uses: | Predicted molecular weight: 29 and 30 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | 14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Mouse |
| Buffer: | Preservative: None Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2 |
| ListPrice: | 285 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Bv, Ca, Hu, Mu, Mk, Rt, Rb, Sh |
| ProteinTarget: | 14-3-3 beta + zeta [22-IID8B] |
| PackageSize: | 0.05 mg |
| GeneralRef: | Choi EK et al. Calsenilin is a substrate for caspase-3 that preferentially interacts with the familial Alzheimer's disease-associated C-terminal fragment of presenilin 2. J Biol Chem 276:19197-204 (2001). Celis A et al. Human proteins IEF 58 and 57a are associated with the Golgi apparatus. FEBS Lett 227:14-20 (1988). General / background references:Aitken A et al. 14-3-3 proteins: biological function and domain structure. Biochem Soc Trans 23:605-11 (1995). Burbelo PD & Hall A 14-3-3 proteins. Hot numbers in signal transduction. Curr Biol 5:95-6 (1995). |
| NotesMain: | Storage below -20 degrees C is not recommended.For maximum product recovery centrifuge the product vial before removing cap. |
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