| CatalogCode: | NB100-1920SS |
| ProductName: | Nucleolin, alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-Nucleolin, alpha **Sample Size** |
| Clonality: | Polyclonal |
| Immunogen: | A 15 residue peptide designed to from C-terminus of the human protein. |
| CrossReactivity: | NB 100-1920 can be used to detect human and rat nucleolin proteins. |
| Packaging: | 0.025 ml Whole antisera Rabbit antisera. |
| Uses: | This antibody is useful for Western blot where a band is seen at ~80 kD. It can also be used for immunocytochemistry (perinuclear staining) and immunoprecipitation. No other applications have been tested.Suggested working dilutions: *Western Blot - 1:10,000-1:20,000 (1-step Western blot kit)**Immunocytochemistry - 1:5,000-1:10,000Immunoprecipitation - 1-3 ug protein (Ammonium sulfate, IgG fraction)*The investigator should determine the optimal dilution for a specific application. ** This GeneScript Corp. system is highly sensitive. |
| Localization: | Nucleoli |
| Background: | Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. It is also a good mammalian nucleolus marker. |
| ProductRef: | 1. Wu, R, et al. J. Cell. Biochem. 77: 507-516 (2000) [WB and EMSA, human] |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host_Name: | Rabbit |
| ListPrice: | 100 |
| AppSummary: | WB, IP, ICC |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-nucl antibody, anti-protein C23 antibody |
| PackageSize: | 0.025 ml |
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