| Catalog Code: | NB100-1915 |
| Product Type: | Primary Antibody |
| Product Name: | Ku70 Antibody |
| List Price: | 295 |
| Clonality: | Monoclonal |
| Gene ID: | 2547 |
| Host: | Mouse |
| Clone: | N3H10 |
| Isotype: | IgG2b |
| Product Description: | Mouse Monoclonal anti-Ku70 (N3H10) |
| Cross Reactivity: | Cross-reacts with Human, Monkey and Xenopus laevis. Cross-reacts with Hamster and Mouse (Western blotting only). Does not cross-react with Cow, Rabbit or Sea Urchin. Not yet tested in other species. |
| Species Summary: | Bv(-), HaWB, Hu, MuWB, Mk, Rb(-), Xp |
| Background: | The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large |
| Alternate Names: | anti-70 kDa subunit of Ku antigen antibody, anti-ATP dependent DNA helicase II 70 kDa subunit antibody, anti-CTC box binding factor 75 kDa subunit antibody, anti-CTC75 antibody, anti-Lupus Ku autoantigen protein p70 antibody, anti-ML8 antibody, anti-Ku 70 |
| Immunogen: | Human placental extract designated as PSE1-PL. |
| Epitope: | Amino acids 506 - 541. |
| Localization: | Nuclear |
| Purity: | protein A purified |
| Buffer: | 10mM PBS [pH7.4] with 0.2%BSA |
| Packaging: | 0.5 ml protein A purified Mouse ascites. |
| Package Size: | 0.5 ml |
| Concentration: | 0.2 mg/ml |
| Uses: | This antibody can be used in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT |
| Application Summary: | IHC, IF, IP, WB |
| General References: | Ajmani AK et al. Absence of autoantigen Ku in mature human neutrophils and human promyelocytic leukemia line (HL-60) cells and lymphocytes undergoing apoptosis. J Exp Med 181:2049-58 (1995). Wang J et al. Murine monoclonal antibodies speci |
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