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| Catalog Code: | NB100-1900 |
| Product Name: | Cofilin Antibody |
| Product Description: | Rabbit Polyclonal anti-Cofilin |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin. |
| Specificity: | Detects a band at approximately 19kDa. |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Chicken, Dog and Pig. Not yet tested in other species. |
| Packaging: | 0.05 ml IgG purified Rabbit antisera. |
| Uses: | This antibody can be used in Immunofluorescence and Western Blot. For IF use NIH-NT3 fibroblasts. For WB use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and dog MDCK kidney cells, or purified recombinant chicken muscle cofilin. Staining of cofilin is inhibited by the immunizing peptide.Not tested in other applications. *Optimal dilutions should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | Cofilin is a small phosphoinositide-sensitive, actinbinding protein capable of depolymerizing actinfilaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 0.01M PBS [pH 7.4] |
| Application Summary: | immunofluorescence 1/1000, Western Blot 1/10000 |
| Species Summary: | Ca, Ch, Hu, Mu, Po, Rt, |
| Alternate Names: | Anti-18 kDa phosphoprotein antibody; Anti-CFL 1 antibody; Anti-CFL antibody; Anti-CFL1 antibody; Anti-Cofilin 1 antibody; Anti-Cofilin 1 non muscle antibody; Anti-Cofilin non muscle isoform antibody; Anti-p18 antibody |
| Package Size: | 0.05 ml |
| Preservative: | Sodium Azide |
| Notes Main: | The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin. |
| General References: | 1. Nishida, E., et al., Biochemistry, 23, 5307-5316 (1984). 2. Nishida, E., et al., Proc. Natl. Acad. Sci. (USA), 84, 5262-5266 (1987). 3. Moriyama, K., and Yahara, I., EMBO J., 18, 6752-6761 (1999). 4. Bamburg, R., Annu. Rev. Cell. Dev. Biol., 15, 185-230 (1999). 5. Carlier, M.F., et al., J. Cell Biol., 136, 1307-1322 (1997). 6. Maciver, S.K., et al., J. Cell. Biol., 278, 1611-1620 (1991). 7. Ohta, Y., et al., J. Biol. Chem., 264, 16143-16148 (1989). 8. Suzuki, K.T., et al., J. Biol. Chem., 270, 19551-19556 (1997). 9. Minamide, A., et al., Nature Cell Biol., 2, 628-636 (2000). 10. Arber, S., et al., Nature, 393, 805-809 (1998). |
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