| CatalogCode: | NB100-1883 |
| ProductName: | Osteopontin Antibody |
| Product Description: | Goat Polyclonal anti-Osteopontin |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: C-DPKSKEEDKHLK, representing the C-terminus region of the human protein according to NP_000573.1. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross react with Mouse, Rat and Dog. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 1-3µg/ml.This antibody has also been used for immunohistochemistry. |
| Localization: | Secreted |
| Control: | Human Thymus, Tonsil and Spleen lysates |
| Background: | Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline containing 0.5% BSA |
| ListPrice: | 295 |
| NovusRef: | Horak, C.E., et al. Nm23-H1 Suppresses Tumor Cell Motility by Down-regulating the Lysophosphatidic Acid Receptor EDG2. Cancer Res. 2007 67: 7238-7246. 2. Alonso, S.R., et al. A High-Throughput Study in Melanoma Identifies Epithelial-Mesenchymal Transition as a Major Determinant of Metastasis. Cancer Res. 2007 67: 3450-3460. |
| AppSummary: | ELISA, IHC, WB, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | anti-SPP1 antibody; anti-secreted phosphoprotein 1 (osteopontin antibody; anti-bone sialoprotein I antibody; anti-early T-lymphocyte activation 1) antibody; anti-HGNC:11255 antibody; anti-BNSP antibody; anti-BSPI antibody; anti-ETA-1 antibody; anti-MGC110940 antibody; anti-OPNantibody; anti-Secreted phosphoprotein-1 (osteopontin antibody; anti-bone sialoprotein) antibody; anti-secreted phosphoprotein 1 antibody |
| ProteinTarget: | Osteopontin |
| PackageSize: | 0.1 mg |
| GeneralRef: | Kolb A, Kleeff J, Guweidhi A, Esposito I, Giese NA, Adwan H, Giese T, Buchler MW, Berger MR, Friess H. Osteopontin Influences the Invasiveness of Pancreatic Cancer Cells and is Increased in Neoplastic and Inflammatory Conditions. Cancer Biol Ther. 2005 Jul 5;4(7) [Epub ahead of print] |
| Gene_Id: | 6696 |
| Omim_ID: | NB600-1043 |