| CatalogCode: | NB100-1781 |
| ProductName: | NANOS1 Antibody |
| Product Description: | Goat Polyclonal anti-NANOS1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: C-SARDGPPGKKLR, representing the C Terminus of the human protein according to NP_955631.1 |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for ELISA and Western Blot. In Western Blot, detects a band of approximately 35-37kDa (calculated MW of 30.2kDa according to NP_955631.1). The observed molecular weight corresponds to earlier findings in literature (Clarke et al, Hum Mol Genet. 2004 Apr 1;13(7):727-39. Epub 2004 Feb 12, PMID: 14962983). Recommended starting dilutions/concentrations: ELISA: 1/64,000; WB: 1.0-3.0ug/ml. Optimal dilutions/concentrations should be determined by the end user. Not tested in other applications. |
| Background: | NANOS1 may regulate the translation of specific mRNAs by forming a complex with PUM2 that associates with the 3' untranslated regions (UTRs) of mRNA targets. Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline containing 0.02% Na azide (pH7.3) with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-NANOS1 antibody, anti-nanos homolog 1 (Drosophila) antibody, anti-NOS1 antibody |
| ProteinTarget: | NANOS1 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev Genes Evol. 2003 Apr;213(3):120-6. Epub 2003 Feb 11. |
| NotesMain: | This antibody is expected to recognise the longer isoform 1 only (NP_955631), ie not isoform 2 (NP_001009553). |
| Gene_Id: | 340719 |
| Omim_ID: | H00140838-B01 |