| CatalogCode: | | NB100-1780 |
| ProductName: | | COMT Antibody |
| Product Description: | | Goat Polyclonal anti-COMT |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide: GDTKEQRILNHVLQC, representing the N Terminus of the human protein according to NP_000745.1; NP_009294.1 |
| CrossReactivity: | | Cross-reacts with Human. Expected to cross-react with Chimp, Mouse, Rat, Dog. Not yet tested in other species. |
| Packaging: | | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | | This antibody can be used for ELISA and Western Blot. In Western Blot, detects a band of approximately 25kDa and 28kDa. The 25kDa band was also observed in Human Liver and Placenta lysates. Calculated MW of 24.5kDa according to NP_009294.1 (S-COMT) and 30.0kDa according to NP_000745.1 (MB-COMT). Recommended starting dilutions/concentrations: ELISA: 1:4,000; WB: 1.0-3.0ug/ml. Optimal dilutions/concentrations should be determined by the end user. Not tested in other applications. |
| Background: | | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Goat |
| Buffer: | | Tris-saline containing 0.02% Na azide (pH7.3) with 0.5% BSA |
| ListPrice: | | 295 |
| AppSummary: | | ELISA, WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-COMT antibody, anti-catechol-O-methyltransferase antibody |
| ProteinTarget: | | COMT |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | Tunbridge EM, Harrison PJ, Weinberger DR. Catechol-o-Methyltransferase, Cognition, and Psychosis: Val(158)Met and Beyond. Biol Psychiatry. 2006 Feb 11; [Epub ahead of print] |
| NotesMain: | | This antibody is expected to recognise an epitope corresponding to aa 1-14 of the soluble isoform of human COMT (S-COMT, NP_009294) and an epitope corresponding to aa 52-65 of the membrane-bound isoform of human COMT (MB-COMT, NP_000745). |
| Gene_Id: | | 1312 |
| Omim_ID: | | NB100-78596 |
| more information: | | company product webpage |