| Catalog Code: | NB100-178 |
| Product Type: | Primary Antibody |
| Product Name: | RAD51D Antibody |
| List Price: | 275 |
| Clonality: | Monoclonal |
| Gene ID: | 5892 |
| Host: | Mouse |
| Clone: | 5B3/6 |
| Isotype: | IgG1 |
| Product Description: | Mouse Monoclonal anti-RAD51D (5B3/6) |
| Cross Reactivity: | NB 100-178 recognizes human Rad51D. It does not work on mouse. Other species have not been tested. NB 100-178 does not cross-react with Rad51B, Rad51C, Rad51, XRCC2, or XRCC3 in Western analysis. |
| Species Summary: | Hu, Mu(-) |
| Background: | Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. RAD51D interac |
| Alternate Names: | anti-RAD51L3 antibody, anti-RAD51-like 3 (S. cerevisiae) antibody, anti-TRAD antibody, anti-R51H3 antibody, anti-HsTRAD antibody, anti-RAD51D antibody, anti-recombination repair protein antibody, anti-DNA repair protein RAD51 homolog 4 antibody |
| Immunogen: | His-tagged hRAD51D, overexpressed in E. coli. |
| Specificity: | NB 100-178 is specific for Rad51D. |
| Purity: | protein A purified |
| Buffer: | PBS at physiological pH |
| Packaging: | 0.1 ml protein A purified Mouse ascites. |
| Package Size: | 0.1 ml |
| Uses: | By Western blot, this antibody recognizes a band at ~40 kDa, representing Rad51D protein. *The investigator should determine the optimal working dilution for a specific application. |
| Application Summary: | IF, WB |
| Storage: | Store at 4C. Do not freeze. |
| General References: | 1.Masson, et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes & Dev. 15: 3296-3307, 2001. (Immunoprecipitation, human) 3. Maacke, H. Autoantibodies in sera of pancreatic cancer patients identify reco |
| Novus References: | 1. Fan, R., et al. Defective Strand Break Repair after DNA Damage in Prostate Cancer Cells: Implications for Genetic Instability and Prostate Cancer Progression. Cancer Res. 64: 8526-8533, 2004. 2. Hinz, J.M., et al. Repression of mutagenesis by Rad51D-m |
| Notes Main: | NB 100-178 is unable to detect endogenous protein of HeLa cell extracts. However, immunoprecipitates of the paralog complex from HeLa cell extracts provide a specific signal in Western blot. The IP's are done using either polyclonal anti-Rad51C, an |
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