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| Catalog Code: | NB100-176 |
| Product Name: | RAD51B Antibody |
| Product Description: | Mouse Monoclonal anti-RAD51B (1 H3/13) |
| Clone: | 1 H3/13 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | His-tagged hRAD51B, overexpressed in E. coli. |
| Specificity: | NB 100-176 is specific for Rad51B. |
| Cross Reactivity: | NB 100-176 recognizes human Rad51B. Does not work in mouse. Other species have not been tested. NB 100-176 does not cross-react with Rad51C, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis. |
| Packaging: | 0.1 ml Ascites Mouse ascites. |
| Uses: | By Western blot, this antibody recognizes a band at ~ 38 kDa. Immunoprecipitation (-) |
| Background: | Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. Rad51B plays a role in meiotic recombination and/or recombinational repair. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Host Name: | Mouse |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu(-) |
| Alternate Names: | anti-R51H2 antibody, anti-RAD51 like protein 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | Sodium Azide |
| Notes Main: | NB 100-176 is unable to detect endogenous protein of HeLa cell extracts. However, immunoprecipitates of the paralog complex from HeLa cell extracts provide a specific signal in Western blot. The IP's are done using either polyclonal anti-Rad51C, anti-Rad51B or anti-XRCC2. |
| General References: | 1.Masson, et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes & Dev. 15:3296-3307, 2001. (Immunoprecipitation, human) 2. Albala, et al. Identification of a Novel Human RAD51 Homolog, RAD51B. Genomics. 46:476-479, 1997. 3. Maacke, H. Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad 51 as a tumour-associated antigen. J. Cancer Res. 128:219-222, 2002. (Immunohistochemistry, western blot, human) 4. Lambert, S. Inactivation of the RAD51 recombination pathway stimulates UV-induced mutagenesis in mammalian cells. Oncogene. 21:4065-4069, 2002. (Hamster) |
| Product References: | 1. Nagaraju, G., Odate, S., Xie, A., and Scully, R. (2006) Differential Regulation of Short- and Long-Tract Gene Conversion between Sister Chromatids by Rad51C, 26, 8075-8086. |
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