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| Catalog Code: | NB100-175 |
| Product Name: | DNA polymerase Iota Antibody |
| Product Description: | Rabbit Polyclonal anti-DNA polymerase Iota |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | A 15-mer peptide in the C-terminal region of human DNA Polymerase Iota, conjugated to KLH. |
| Specificity: | This antibody is specific for human DNA Polymerase Iota. |
| Cross Reactivity: | Human. Others not tested. |
| Packaging: | 0.05 ml Whole Rabbit antisera. |
| Uses: | This antibody can be used in Western blot where a band is seen at ~80 kDa representing DNA Polymerase iota. |
| Localization: | Nuclear |
| Background: | DNA polymerase Iota (pol iota, also known as Rad30B) is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in poli. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 1:1000 |
| Species Summary: | Hu |
| Alternate Names: | anti-hRAD30B antibody; anti-RAD30B antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.02% sodium azide |
| General References: | 1. Tissier, A. et al. poli, a remarkably error-prone human DNA polymerase. Genes & Dev. 14:1642-1650, 2000. (Drosophila) 2. Tissier, A. et al. Misinsertion and bypass of thymine-thymine dimmers by human DNA polymerase i. EMBO. 19:5259-5266, 2000. 3. Frank, E.G. et al. Altered nucleotide misinsertion fidelity associated with pol iota-dependent replication at the end of a DNA template. EMBO. 20: 2914-2922, 2001. (Northern Blot, Mouse) 4. Vaisman, A. Human DNA polymerase i promiscuous mismatch extension. The J. of Biological Chem. 276(33):30615-30622, 2001. (Mouse, Human) |
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