| CatalogCode: | NB100-1699 |
| ProductName: | HEF1 Antibody |
| Product Description: | Mouse Monoclonal anti-HEF1 (2G9) |
| Clone: | 2G9 |
| Clonality: | Monoclonal |
| Immunogen: | Fusion protein, corresponding to amino acids 82-398 of Human HEF1. |
| Specificity: | Does not recognize p130 Cas. Not tested on Sin1.This antibody mostly detects HEF1 localized at the focal adhesion sites. |
| CrossReactivity: | Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | IF: Performed on 4% PFA fixed cells.WB: Predicted molecular weight: 93 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle. |
| Control: | MCF7, Hela, SKMEL5 (human), NIH 3T3, MEF (mouse), 3Y1 (rat) and CHO (hamster) cell lines. |
| Storage: | Store at -20 degrees C or below. |
| Purity: | protein A purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | 0.2 M Potassium phosphate [pH 7.2], 0.15 M NaCl |
| ListPrice: | 325 |
| AppSummary: | IF, IP, WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ProteinTarget: | HEF-1 |
| PackageSize: | 0.1 mg |
| GeneralRef: | O'Neill GM & Golemis EA Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics. Mol Cell Biol 21:5094-108 (2001). |
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