| CatalogCode: | NB100-1641 |
| ProductName: | Aurora A Antibody |
| Product Description: | Mouse Monoclonal anti-Aurora A (35C1) |
| Clone: | 35C1 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length his-tagged Aurora A protein (Human). |
| Specificity: | The specificity of this antibody was confirmed by showing that it binds in ELISA to recombinant Aurora A, it detects a 46kd band on HeLa cell extract and it binds to duplicated centrosomes and spindle poles in MCF7 cells. This antibody does not inhibit the kinase activity of Aurora A.This antibody has been successfully used to detect Aurora A in the following cell extracts: 293T, HMEC, T47D, MCF7, MDA-MB-468, SK-Br-3, S68 and HeLa. |
| CrossReactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.05 mg protein G purified Mouse ascites. |
| Uses: | This antibody can be used in Western Blot, Immunocytochemistry, Immunofluorescence, ELISA, and Immunoprecipitation. In Western Blot a 46 kDa band is seen.*Optimal dilutions should be determined by the end user. |
| Background: | NB 100-1641 recognizes human Aurora-A kinase, a 46kDa serine-threonine kinase involved in mitotic spindle assembly. Aurora-A is reported to be overexpressed in many epitheial cancers and is thought to play an important for in tumorgenesis. |
| ProductRef: | Cremet JY et al. Preparation and characterization of a human aurora-A kinase monoclonal antibody. Mol Cell Biochem 243:123-31 (2003). |
| Purity: | protein G purified |
| Isotype: | IgG2b |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | ELISA, IF, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-AIK antibody, anti-ARK1 antibody, anti-AURA antibody, anti-Aurora 2 antibody, anti-Aurora A antibody, anti-Aurora Family Kinase 1 antibody, anti-Aurora Related Kinase 1 antibody, anti-Aurora/IPL1 Like Kinase antibody, anti-Aurora/IPL1 Related Kinase 1 antibody, anti-AYK1 antibody, anti-BRAK antibody, anti-Breast Tumor Amplified Kinase antibody, anti-BTAK antibody, anti-IAK antibody, anti-IPL1 Aurora Related Kinase 1 antibody, anti-IPL1 Related Kinase antibody, anti-Serine/threonine kinase 15 antibody, anti-Serine/threonine kinase 6 antibody, anti-Serine/threonine kinase antibody, anti-STK15 antibody, anti-STK6 antibody |
| ProteinTarget: | Aurora A [35C1] |
| PackageSize: | 0.05 mg |
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