| CatalogCode: | NB100-1506 |
| ProductName: | GAD65 Antibody |
| Product Description: | Goat Polyclonal anti-GAD65 |
| Clonality: | Polyclonal |
| Immunogen: | Peptide with sequence CTLEDNEERMSRLSK, from the Internal of the protein sequence according to NP_000809.1. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. Expected to react with mouse, rat, dog and chimpanzee based on sequence similarity. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Suggested Starting Dilutions:*ELISA (peptide): detection limit dilution 1:32,000. Western Blot: 0.3-1ug/ml. ~65+70kDa bands observed in Human Brain lysates. An additional band of 26kDa is observed. All bands were blocked by the immunising peptide (calculated MW of 65.4kDa according to NP_000809.1)*Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | unknown |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-DCE 2 antibody, anti-DCE2 antibody, anti-GAD 2 antibody, anti-GAD 65 GAD-2 antibody, anti-GAD-65 antibody, anti-GAD2 antibody, anti-Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody, anti-Glutamate Decarboxylase 2 antibody, anti-Glutamate Decarboxylase 65 antibody, anti-Glutamic Acid Decarboxylase 2 antibody, anti-Glutamic Acid Decarboxylase 65 antibody |
| ProteinTarget: | GAD65 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Meyre D, et al. Is Glutamate Decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children? J Clin Endocrinol Metab. (2005)[Epub ahead of print] PMID: 15671113 |
| Gene_Id: | 2572 |
| Omim_ID: | NB110-41528 |