| CatalogCode: | | NB100-150 |
| ProductName: | | FEN1 Antibody |
| Product Description: | | Mouse Monoclonal anti-FEN1 (4E7) |
| Clone: | | 4E7 |
| Clonality: | | Monoclonal |
| Immunogen: | | Recombinant human FEN-1 protein encoding amino acids 1-380 purified from baculovirus-infected Sf-9 cells. |
| Specificity: | | This antibody is specific for FEN-1. |
| CrossReactivity: | | NB 100-150 reacts with human FEN-1. |
| Packaging: | | 0.1 mg protein G purified Mouse ascites. |
| Uses: | | Immunoblotting |
| Control: | | MCF-7 cells, recombinant fusion protein. |
| Background: | | FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | protein G purified |
| Isotype: | | IgG1/kappa |
| Host_Name: | | Mouse |
| Buffer: | | PBS [pH 7.4] |
| ListPrice: | | 285 |
| NovusRef: | | 1. Qiu, J., et al. Cell Cycle-dependent and DNA Damage-inducible Nuclear Localization of FEN-1 Nuclease Is Consistent with Its Dual Functions in DNA Replication and Repair. The Journal of Biological Chemistry. 276(7): 4901-4908, 2001. 2. Shibata, Y., Nakamura, T. Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay. J. Biol. Chem. 277:746-754, 2001. 3. Kedar, P.S. et al. Direct interaction beween mammalian DNA polymerase beta and prolifeating cell nuclear antigen. J. Biol. Chem. 277:31115-31123, 2002. 4. Kundu, C.N. et al. CANCER BIOLOGY: Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways. Carcinogenesis. 28:2089-2095, Oct 2007 |
| AppSummary: | | FACS, IF, WB, ICC |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-Flap endonuclease 1 antibody, anti-flap structure specific endonuclease 1 antibody, anti-Maturation factor 1 antibody, anti-MF1 antibody, anti-RAD2 (S. pombe) homolog antibody, anti- fen-1 antibody |
| ProteinTarget: | | FEN-1 |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | 1.Kolodner, R.D. and Marsischky, G.T. (1999) Eukaryotic DNA mismatch repair. Curr Opin Genet Dev 9:89-96. 2.Rumbaugh, J.A., Henricksen, L.A., DeMott, M.S. and Bambara, R.A. (1999) Cleavage of substrates with mismatched nucleotides by flap endonuclease-1. Implications for mammalian Okazaki fragment processing. J Biol Chem 274:14602-8 3.Kim, K., Biade, S. and Matsumoto,Y. (1998) Involvement of flap endonuclease 1 in base excision DNA repair. J Biol Chem 273:8842-8848. 4.Warbrick, E., Coates, P.J. and Hall, P.A. (1998) Fen1 expression: a novel marker for cell proliferation. J Pathology 186:319-24. 5.Hiraoka, L., Harrington, J., Gerhard, D.S., Lieber, M.R. and Hsieh, C.L. (1995) Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. Genomics 25:220-5. |
| NotesMain: | | Hybridoma: Fusion between BALB/c splenocytes and mouse myeloma (NS-1 cells). |
| more information: | | company product webpage |
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