| Catalog Code: | NB100-148 |
| Product Type: | Primary Antibody |
| Product Name: | RAD51 Antibody |
| List Price: | 285 |
| Clonality: | Monoclonal |
| Gene ID: | 5888 |
| Host: | Mouse |
| Clone: | 14B4 |
| Isotype: | IgG2bkappa |
| Product Description: | Mouse Monoclonal anti-RAD51 (14B4) |
| Cross Reactivity: | NB 100-148 reacts with human Rad51. |
| Species Summary: | Hu |
| Background: | Rad51, a 37kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BR |
| Alternate Names: | anti-DNA repair protein RAD51 homolog 1 antibody, anti-E coli RecA homolog antibody, anti-HGNC:9817 antibody, anti-Homolog of E coli RecA antibody, anti-homolog of S cerevisiae RAD51 antibody, anti-HRAD51 antibody, anti-HsRad51 antibody, anti-HsT16930 ant |
| Immunogen: | Fusion protein encoding full length (amino acids 1-338) of Rad51 expressed in E. coli. |
| Specificity: | This antibody is specific for Rad51. |
| Purity: | protein G purified |
| Buffer: | PBS [pH7.4] |
| Packaging: | 0.1 mg protein G purified Mouse ascites. |
| Package Size: | 0.1 mg |
| Concentration: | 1 mg/ml |
| Uses: | Immunoblotting, immunofluorescence, and immunoprecipitation. Suggested working dilutions: * Western Blot 1-5 ug/ml Immunofluorescence 2-10 ug/ml Immunoprecipitation 1-5 ug/ml *The investigator should determine the optimal working dilution for a specific a |
| Application Summary: | WB, IP, IF |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| General References: | 1.Shinohara, A., Ogawa, H., Matsuda, Y., Ushio, N., Ikeo, K. and Ogawa, T. (1993) Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet 4(3):239-43. 2. Scully, R., Chen , J., Plug, A., Xiao, Y., Weaver, D., |
| Product References: | 1. Tarsounas, M., et al. BRCA2-dependent and independent formation of RAD51 nuclear foci. Oncogene. 22:1115-1123, 2001. 2. French, C.A., et al. Role of Mammalian RAD51L2 (RAD51C) in recombination and genetic stability. J. Biol. Chem. 277:19322-19330, 2002 |
| Novus References: | 1. Shim, K., et al. hXRCC2 Enhances ADP/ATP Processing and Strand Exchange by hRAD51. The Journal of Biological Chemistry. 279(29):30385-30394, 2004.2. Stewart, G.S. et al. CELL BIOLOGY: RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-media |
| Notes Main: | Hybridoma: Fusion between Balb/c splenocytes and mouse myeloma (NS-1 cells). |
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