| CatalogCode: | NB100-146 |
| ProductName: | FEN1 Antibody |
| Product Description: | Mouse Polyclonal anti-FEN-1 (FEN-1) |
| Clone: | FEN-1 |
| Clonality: | Polyclonal |
| Immunogen: | Recombinant human FEN-1 protein encoding amino acids 1-380 purified from baculovirus infected Sf9 cells. |
| Specificity: | This antibody is specific for FEN-1. |
| CrossReactivity: | NB 100-146 reacts with human FEN-1. |
| Packaging: | 0.05 ml Whole antisera Mouse antisera. |
| Uses: | Immunoblotting. HepG2, HeLa, T24, and HBL100 cell lysates have been tested.Suggested working dilutions: *Western Blot 1:500~1:1000*The investigator should determine the optimal working dilution for a specific application. |
| Control: | Recombinant fusion protein |
| Background: | FEN-1 (Flap EndoNuclease and Five prime Exonuclease) is a 42kDa protein that functions as an endonuclease and cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair, and is required in cell-free DNA replication systems. |
| Storage: | Aliquot and store at -20C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host_Name: | Mouse |
| ListPrice: | 295 |
| NovusRef: | 1. Wang, W., et al. The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1. PNAS. 101(48): 16762-16767, 2004. (Immunoprecipitation, Western blot) |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Flap endonuclease 1 antibody, anti-flap structure specific endonuclease 1 antibody, anti-Maturation factor 1 antibody, anti-MF1 antibody, anti-RAD2 (S. pombe) homolog antibody, anti-FEN-1 antibody |
| ProteinTarget: | FEN-1 |
| PackageSize: | 0.05 ml |
| GeneralRef: | 1.Hiraoka, L.R., Harrington, J.J., Gerhard, D.S., Lieber, M.R. and Hsieh, C.L. (1995) Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. Genomics 25:220-5. 2.Warbrick, E., Coates, P.J. and Hall, P.A. (1998) Fen1 expression: a novel marker for cell proliferation. J Pathol 186:319-24. 3.Kim, K., Biade, S. and Matsumoto,Y. (1998) Involvement of flap endonuclease 1 in base excision DNA repair. J Biol Chem 273:8842-8848. 4.Rumbaugh, J.A., Henricksen, L.A., DeMott, M.S. and Bambara, R.A. (1999) Cleavage of substrates with mismatched nucleotides by flap endonuclease-1. Implications for mammalian Okazaki fragment processing. J Biol Chem 274:14602-8. 5.Kolodner, R.D. and Marsischky, G.T. (1999) Eukaryotic DNA mismatch repair. Current Opinion Genet Dev 9:89-96. |
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