| CatalogCode: | | NB100-1448 |
| ProductName: | | 58K Golgi protein Antibody |
| Product Description: | | Goat Polyclonal anti-58K Golgi protein |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide: C-LREQGRGKDQPGRL, from the internal region of the protein sequence according to NP_006648; NP_996848. |
| Specificity: | | ab23932 is expected to recognise both reported isoforms. |
| CrossReactivity: | | Reacts with Human.Predicted to react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Chicken (84% identity with immunogen), Zebrafish (90% identity with immunogen) and Pig (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | | This antibody can be used for ELISA and Western Blot. In Western Blot, predicted molecular weight: 59 kDa. Not tested in other applications.Recommended Starting Dilution:*ELISA (Peptide): 1/32000WB: 0.03 - 0.1 ug/ml*Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Cytoplasmic and Golgi Apparatus |
| Background: | | 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Goat |
| Buffer: | | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | | 295 |
| AppSummary: | | ELISA, WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-Formiminotransferase cyclodeaminase antibody, anti-FTCD antibody, anti-Glutamate formiminotransferase antibody, anti-LCHC1 antibody |
| ProteinTarget: | | 58K Golgi protein |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | Bloom GS & Brashear TA A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem 264:16083-92 (1989). |
| Gene_Id: | | 10841 |
| Omim_ID: | | NB100-1447 |
| more information: | | company product webpage |