| CatalogCode: | NB100-1423 |
| ProductName: | ACTR1A Antibody |
| Product Description: | Goat Polyclonal anti-ACTR1A |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: YEEDGARSIHRKT representing the C-terminus of the human protein (residues 363-375) according to NP_005727. |
| Specificity: | This antibody may crossreact with ACTR1B |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen) and Rat due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for ELISA and Western Blot. In Western Blot, detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa). Not tested in other applications.Recommended Starting Dilution:*WB: 1 - 3 ug/mlELISA (peptide): 1/8000*Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline containing 0.02% Na azide (pH7.3) with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | WB, ELISA |
| SpeciesSummary: | Hu |
| ALTnames: | anti-actin related protein 1 antibody, anti-Actin RPV antibody, anti-Alpha Centractin antibody, anti-ARP1 antibody, anti-Centrosome associated actin homolog antibody |
| ProteinTarget: | ACTR1A |
| PackageSize: | 0.1 mg |
| GeneralRef: | Lees-Miller JP et al. A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 359:244-6 (1992). |
| NotesMain: | GenBank Accession Number - NP_005727 |
| Gene_Id: | 10121 |
| Omim_ID: | H00010121-A01 |
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