| CatalogCode: | NB100-1393 |
| ProductName: | STK35 Antibody |
| Product Description: | Goat Polyclonal anti-STK35 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide ELETRMDQVTCAA, representing the C-Terminus of the human protein (residues 389-401), according to NP_543026. |
| CrossReactivity: | Reacts with Human. Predicted to react with Mouse (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for ELISA and Western Blot. In Western Blot, approx 45kDa band observed in Human Testis lysates (calculated MW of 44.6kDa according to NP_543026. This antibody has also been used for immunohistochemistry. Not tested in other applications. Recommended Starting Dilution:*ELISA (peptide): 1:16,000WB: 1-3ug/ml*Optimal dilutions should be determined by the end user. |
| Background: | STK35 associates with the PDZ-LIM protein CLP36 through the LIM domain and undergoes autophosphorylation in vitro. STK35 localizes predominantly to the nucleus; however, cotransfection of STK35 with CLP36 demonstrated redistribution of STK35 to the cytoplasm and into actin stress fibers, where it colocalizes with CLP36. The presence of STK35 disrupted the periodic staining pattern of CLP36 in these fibers. It is suggested that CLP36 specifically targets STK35 to actin stress fibers and that STK35 may represent a regulator of the actomyosin cytoskeleton in nonmuscle cells. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline containing 0.02% Na azide [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, IHC, WB, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Clik1 antibody, anti-CLP-36 interacting kinase antibody, anti-Serine/threonine protein kinase 35 antibody |
| ProteinTarget: | STK35 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Vallenius T & Mkel TP Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein. J Cell Sci 115:2067-73 (2002). PubMed: 11973348 |
| Gene_Id: | 140901 |
| Omim_ID: | H00027148-B01 |