| CatalogCode: | NB100-136 |
| ProductName: | CITED2 Antibody |
| Product Description: | Mouse Monoclonal anti-CITED2 (JA22) |
| Clone: | JA22 |
| Clonality: | Monoclonal |
| Immunogen: | Human CITED 2 protein. |
| Specificity: | This antibody is specific for CITED 2. |
| CrossReactivity: | Human. Does not work well on mouse. Other species have not been tested. |
| Packaging: | 0.1 ml Ascites Mouse ascites. |
| Uses: | This nuclear antibody may be used for Western blotting where a band is seen at 29 kDa, representing CITED 2.Additional bands may be seen at 36 kDa representing p35srj, the alternativly spliced version of CITED2(MRG1), and at 70 kDa (unknown)**. Does not work well on mouse tissue.Immunohistochemistry and Immunoprecipitation has not been tested for this lot. *The investigator should determine the optimal working dilution for a specific application.** See Product Reference 1. |
| Localization: | Nuclear |
| Background: | Cited 2 is a cAMP-responsive element-binding protein (CBP)/p300 interacting transcriptional modulator and a proposed negative regulator for hypoxia-inducible factor-1 alpha. Recruitment of CBP/p300 by HIF-1 is essential for the transcriptional response to hypoxia. |
| ProductRef: | 1. Bhattacharya, S., et al. Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-Genes Dev. 13(1): 64-75, 1999. 2. Haase, M., Schott, M., Bornstein, S. R., Malendowicz, L. K., Scherbaum, W. A., and Willenberg, H. S. (2007) CITED2 is expressed in human adrenocortical cells and regulated by basic fibroblast growth factor, 192, 459-465. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| ListPrice: | 285 |
| NovusRef: | 1. Tien, E., et al. Identification of the CREB-binding Protein/p300-interacting Protein CITED2 as a Peroxisome Proliferator-activated Receptor alpha Coregulator. J. Biol. Chem. 279: 24053-24063, 2004. 2. Bamforth, S., et al. Cited2 controls left-right patterning and heart development through a Nodal-Pitx2c pathway. Nature Genetics. 36: 1189-1196, 2004. 3. Dabir P, Marinic TE, Krukovets I, Stenina OI. Aryl Hydrocarbon Receptor Is Activated by Glucose and Regulates the Thrombospondin-1 Gene Promoter in Endothelial Cells. Circ Res. May 30, 2008:CIRCRESAHA.108.176990. |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-CITED2 antibody; anti-Cbp/p300-interacting transactivator antibody; anti-with Glu/Asp-rich carboxy-terminal domain antibody; anti-2antibody; anti-HGNC:1987 antibody; anti-MRG1 antibody; anti-P35SRJ antibody; anti-CITED 2 antibody |
| ProteinTarget: | CITED2 |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Leung, M-K., et al. Molecular cloning and chromosomal localization of the human CITED2 gene encoding p35srj/Mrg1. Genomics. 61: 307-313, 1999. 2. Yin, Z., et al. The essential role of Cited 2, a negative regulator for HIF-1 alpha, in heart development and neurulation. PNAS. 99(16): 10488-10493, 2002. |
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