| CatalogCode: | NB100-130 |
| ProductName: | HIF-1 alpha Antibody |
| Product Description: | Mouse Monoclonal anti-HIF-1 alpha (H1alpha 67-7) |
| Clone: | H1alpha 67-7 |
| Clonality: | Monoclonal |
| Immunogen: | Fusion protein containing amino acids 432-528 of human HIF-1 alpha. |
| Specificity: | This antibody is specific for HIF-1 alpha.(1) |
| CrossReactivity: | NB 100-130 recognizes human. Other species have not been tested. |
| Packaging: | 0.1 ml protein G purified Mouse ascites. |
| Uses: | By Western blot, this antibody recognizes bands at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 120 kDa representing post-translational modification of HIF-1 alpha. NB 100-130 has also been used to immunoprecipitate human HIF-1 alpha.(1). *The investigator should determine the optimal working dilution for a specific application. Nuclear extracts are recommended. ** NB 100-123 or NB 100-131 is recommended for immunohistochemical analysis of HIF-1alpha. |
| Localization: | Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. |
| Control: | NB800-PC26 |
| Background: | Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets. |
| ProductRef: | 1. Personal communications with Dr. Gregg Semenza, Johns Hopkins. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Host_Name: | Mouse |
| ListPrice: | 285 |
| NovusRef: | Veschini, L., et al. Hypoxia-inducible transcription factor-1 alpha determines sensitivity of endothelial cells to the proteosome inhibitor bortezomib. Blood, Mar 2007; 109:2565 - 2570. |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Hypoxia-inducible factor 1 alpha antibody; anti-HIF1 alpha antibody; anti-ARNT interacting proteinantibody; anti-Hif1a antibody; anti-ARNT interacting protein antibody; anti-HIF-1alpha antibody; anti-Hypoxia inducible factor 1 alpha antibody; anti-Hypoxia inducible factor 1 alpha subunit basic helix antibody |
| ProteinTarget: | HIF-1 alpha |
| PackageSize: | 0.1 ml |
| NotesMain: | Lot A is 1.4 mg/ml Lot B is 1.5 mg/ml * The mobility of HIF-1 alpha induced by desferrioxamine or cobalt chloride treatment differs from the mobility of the hypoxia-induced protein. The reason is not known. |
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