| CatalogCode: | NB100-128 |
| ProductName: | Aryl hydrocarbon Receptor Antibody |
| Product Description: | Goat Polyclonal anti-Aryl hydrocarbon Receptor |
| Clonality: | Polyclonal |
| Immunogen: | N-terminal sequence of the Ah receptor based on the Ah receptor purified from C57BL/6J mice. |
| Specificity: | This antibody is specific for AHR. |
| CrossReactivity: | Human and Mouse. Other species not tested. |
| Packaging: | 1 ml Whole antisera Goat antisera. |
| Uses: | Suggested working dilution for gel shift: add 4 microliter AhR-AB to a 15 ul final volume of EMSA sample.Other applications have not been tested. |
| Background: | The Ah Receptor belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. All of these proteins share an N-terminal sequence of ~200 amino acids termed the PAS domain. This receptor, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. |
| ProductRef: | 1. Poland, A., Glover, E., and C.A. Bradfield. Characterization of polyclonal antibodies to the Ah receptor prepared by immunization with a synthetic peptide hapten. Molecular Pharmacology. 39: 20-26, 1991. 2. Chan, W.K., Ruiyin, C., Sanjay, J., Reddy, J.K., and C.A. Bradfield. Baculovirus Expression of the Ah Receptor and Ah Receptor Nuclear Translocator. Journal of Biological Chemistry. 269(42): 26464-26474, 1994. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Goat |
| ListPrice: | 285 |
| NovusRef: | 1. Minsavage, G.D., et al. Mutational analysis of the mouse aryl hydrocarbon receptor tyrosine residues necessary for recognition of dioxin response elements. Archives of Biochemistry and Biophysics. 412:95-105, 2003. 2. Tsuchiya, Y: Critical Enhancer Region to Which AhR/ARNT and Sp1 Bind in the Human CYP1B1 Gene: Journal of Biochemistry. 133, 583-592(2003) 3. Hayashibara, T: Possible involvement of arcyl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL. Biochemical and Biophysical research Communications 300 (2003)128-134. 4. Fisher, M., et al. Combined Screening of Thymocytes Using Apoptosis-Specific cDNA Array and Promoter Anylysis Yields Novel Gene Targets Mediating TCDD-Induced Toxicity. Toxicological Sciences. 78:116-124, 2004. 5. Dabir P, Marinic TE, Krukovets I, Stenina OI. Aryl Hydrocarbon Receptor Is Activated by Glucose and Regulates the Thrombospondin-1 Gene Promoter in Endothelial Cells. Circ Res. May 30, 2008:CIRCRESAHA.108.176990. |
| AppSummary: | GS |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-Dioxin Receptor antibody; Anti-AhR antibody |
| ProteinTarget: | AhR |
| PackageSize: | 1 ml |
| GeneralRef: | 1. Jain, S. et al. Potent transactivation domains of the Ah receptor and the Ah receptor nuclear translocator map to their carboxy termini. Journal of Biological Chemistry. 269 (50): 31518-24, 1994. 2. Schmidt, J.V., et al. Characterization of a murine Ahr null allele: Involvement of the Ah receptor in hepatic growth and development. Proc. Natl. Acad. Sci. USA. 93: 6731-6736, 1996. |
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