| CatalogCode: | NB100-124 |
| ProductName: | HIF-1 Beta Antibody |
| Product Description: | Mouse Monoclonal anti-HIF-1 Beta (H1beta234) |
| Clone: | H1beta234 |
| Clonality: | Monoclonal |
| Immunogen: | Fusion protein containing amino acids 496-789 of human HIF-1 beta. |
| Specificity: | This antibody is specific for HIF 1 beta /ARNT. |
| CrossReactivity: | Human, bovine, sheep, mouse, rat and ferret. Other species have not been tested. |
| Packaging: | 0.1 ml protein G purified Mouse ascites. |
| Uses: | This antibody is useful for Western blot and immunohistochemistry. By Western blot a band at approximately 92 kDa is seen. |
| Background: | Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. |
| ProductRef: | 1. Personal communications with G. Semenza and E. Laughner, Johns Hopkins University. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 285 |
| NovusRef: | 1. Alam, H., Follicle-stimulating Hormone Activation of Hypoxia-inducible Factor-1 by the Phosphatideylinositol 3-Kinase/AKT/Ras Homolog Enriched in Brain (Rheb)/ Mammalian Target of Rapamycin (mTOR) Pathway Is Necessary for Induction of Select Protein Markers of Follicular Differentiation. Mechanisms of Signal Transcution. 279: 19431-19440 (2004). 2. Amir, S., et al. MSF-A Interacts with Hypoxia-Inducible Factor-1{alpha} and Augments Hypoxia-Inducible Factor Transcriptional Activation to Affect Tumorigenicity and Angiogenesis. Cancer Research. 66: 856-866 (2006). 3. Arsham, A., et al. Akt and Hypoxia-Inducible Factor-1 Independently Enhance Tumor Growth and Angiogenesis. Cancer Research. 64: 3500-3507, (2004). 4. Asikainen, T.M., et al. Improved lung growth and function through hypoxia-inducible factor in primate chronic lung disease of prematurity. FASEB J. 20: 1698-1700 (2006). 5. Bernaudin, M., et al. Brain Genomic Response Following Hypoxia and Re-oxygenation in the Neonatal Rat: Identification of Genes That Might Contribute to Hypoxia-Induced Ischemic Tolerance. J. Biol. Chem. 277(42): 39728-39738 (2002). 6. Elvert, G., et al. Cooperative Interaction of Hypoxia-inducible Factor-2 alpha (HIF-2 alpha) and Ets-1 in Transcriptional Activation of Vascular Endothelial Growth Factor Receptor-2 (Flk-1). J. of Biol. Chem. 278(9): 7520-7530 (2003). 7. Fukuda, R., et al. IGF-1 Induces HIF-1-mediated VEGF Expression that is Dependent on MAP Kinase and PI-3-Kinase Signaling in Colon Cancer Cells. JBC Papers in Press. 277(41): 38205-38211 (2002). 8. Fukuda, R., et al. Vascular Endothelial Growth Factor Gene Expression in Colon Cancer Cells Exposed to Prostaglandin E2 Is Mediated by Hypoxia-inducible Factor 1. Cancer Research. 63: 2330-2334 (2003) 9. Hirota, K., et al. Induction of Hypoxia-inducible Factor 1 Activity by Muscarinic Acetylcholine Receptor Signaling. J. of Biol. Chem. 279(40): 41521-41528 (2004). 10. Hu, C-J., et al. Differential regulation of the transcriptional activities of hypoxia-inducible factor 1 alpha (HIF-1) and HIF-2 in stem cells. Mol. Cell Biol. 26: 3514-3526 (2006). 11. Hui, A. S., et al. Calcium signaling stimulates translation of HIF-{alpha} during hypoxia. FASEB J. 20: 466-475 (2006). 12. Kasuno, K., et al. Nitric Oxide Induces Hypoxia-inducible Factor 1 Activation That Is Dependent on MAPK and Phosphatidylinositol 3-Kinase Signaling. J. of Biol. Chem. 279(4): 2550-2558 (2004). 13. Mabjeesh, N., et al. 2ME2 inhibits tumor growth and angiogenesis by disrupting microtubules and dysregulating HIF. Cancer Cell. 3: 363-375 (2003). 14. Mabjeesh, N., et al. Geldanamycin Induces Degradation of Hypoxia-inducible Factor 1alpha Protein via the Protesome Pathway in Prostate Cancer Cells. Cancer Research. 62: 2478-2482 (2002). 15. Mabjeesh, N. Dibenzoylmethane, a natural dietary compound, induces HIF-1alpha and increases expression of VEGF. Biochem. and Biophys. Res. Comm. 303: 279-286 (2003). 16. MSF-A Interacts with Hypoxia-Inducible Factor-1alpha and Augments Hypoxia-Inducible Factor Transcriptional Activation to Affect Tumorigencity and Angiogenesis. Cancer Res. 66:(2) (2006). 17. Nakamura, K., et al. Brain-Derived Neurotrophic Factor Activation of TrkB Induces Vascular Endothelial Growth Factor Expression via Hypoxia-Inducible Factor-1{alpha} in Neuroblastoma Cells. Cancer Research. 66: 4249-4255 (2006). 18. Oda, T., et al. Activation of hypoxia-inducible factor 1 during macrophage differentiation. Am. J. Physiol. Cell Physiol. 291: C104-C113 (2006). 19. Okuyama, H., et al. Expression of Vascular Endothelial Growth Factor Receptor 1 in Bone Marrow-derived Mesenchymal Cells Is Dependent on Hypoxia-inducible Factor 1. J. Biol. Chem. 281(22): 15554-15563 (2006). 20. Rapisarda, A., et al. Topoisomerase I-Mediated Inhibition of Hypoxia-Inducible Factor 1. Mechanisms and Therapeutic Implications. Cancer Research. 64: 1475-1482 (2004). 21. Rapisarda, A., et al. Identification of Small Molecule Inhibitors of Hypoxia-Inducible Factor 1 Transcriptional Activation Pathway. Cancer Research. 62: 4316-4324 (2002). 22. Schultz, K., et al. Hypoxia and hypoxia-inducible factor-1 alpha promote growth factor-induced proliferation of human vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 290: H2528-2534 (2006). 23. Slomiany, M. G., and Rosenzweig, S. A. Hypoxia-Inducible Factor-1-Dependent and -Independent Regulation of Insulin-Like Growth Factor-1-Stimulated Vascular Endothelial Growth Factor Secretion. 318: 666-675 (2006). 24. Slomiany, M., et al. Autocrine effects of IGF-I-induced VEGF and IGFBP-3 secretion in retinal pigment epithelial cell line ARPE-19. Am. J. Physiol. Cell Physiol. 287: 746-753 (2004). 25. Slomiany, M., et al. IFG-1-Induced VEGF and IFBP-3 Secretion Correlates with Increased HIF-1 alpha Expression and Activity in Retinal Pigment Epithelial Cell Line D407. Invest. Ophthalmol. Vis. Sci. 45: 2838-2847 (2004). 26. Tendler, D., et al. Intersction of Interferon and Hypoxia Signal Transduction Pathways in Nitric Oxide-induced Tumor Apoptosis. Cancer Research. 61: 3682-3688 (2001). 27. van Patot, M.C., et al. Greater vascularity, lowered HIF-1/DNA binding, and elevated GSH as markers of adaptation to in vivo chronic hypoxia. Am. J. Physiol. Lung Cell Mol. Physiol. 287: 525-532 (2004) 28. Volm, M., et al. Protein expression profiles indicative for drug resistance of non-small cell lung cancer. British J. of Cancer. 87: 251-257 (2002). 29. Zagzag, D., et al. Expression of hypoxia-inducible factor 1alpha in human brain tumors: association with angiogenesis, invasion, and progression. Cancer. 88: 2606-2618 (2000). 30. Zhang, H., et al. HIF-1 Inhibits Mitochondrial Biogenesis and Cellular Respiration in VHL-Deficient Renal Cell Carcinoma by Repression of C-MYC Activity. Cancer Cell 11: 407-420 (2007). 31. Zhong, H., et al. Hypoxia-inducible Factor 1 as a novel diagnostic and therapeutic target in human Cancers. Recent Res. Devel. Cancer. 3: 49-68 (2001). 32. Zhong, H., et al. Hypoxia-Inducible Factor 1alpha and 1beta Proteins Share Common Signaling Pathways in Human Prostate Cancer Cells. Biochem. and Biophys. Res. Comm. 284: 352-356 (2001). 33. Baek, J.H. et al. Spermidine/Spermine N1-Acetyltransferase-1 Binds to Hypoxia-inducible Factor-1alpha (HIF-1alpha) and RACK1 and Promotes Ubiquitination and Degradation of HIF-1alpha. J. Biol. Chem. 282(43): 33358-33366 (2007). 34. Baek, J.H., et al. Spermidine/Spermine-N1-Acetyltransferase 2 Is an Essential Component of the Ubiquitin Ligase Complex That Regulates Hypoxia-inducible Factor 1 alpha. J. Biol. Chem. 282(32): 23572-23580 (2007). 35. Engebretsen, B., et al. Acute Hypobaric Hypoxia (5486 m) Induces greater Pulmonary HIF-1 Activation in Hilltop Compared to Madison Rats. High Altitude Medicine and Biology. 8(4): 312-321, 2007. 36. Cheng G, Shao Z, Chaudhari B, Agrawal DK. Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2007 November 1, 2007;293(5):L1339-47. |
| AppSummary: | WB, IHC-P |
| SpeciesSummary: | Bv, Hu, Mu, Rt, Ft, Sh |
| ALTnames: | anti-ARNT antibody, anti-Aryl Hydrocarbon Receptor Nuclear Translocator antibody, anti-Dioxin Receptor antibody, anti-Hypoxia Inducible Factor 1 antibody, anti-Tango antibody, anti-HIF1 beta antibody, anti-HIF1beta antibody |
| ProteinTarget: | HIF-1 Beta |
| PackageSize: | 0.1 ml |
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