| CatalogCode: | NB100-1195 |
| ProductName: | ALMS 1 Antibody |
| Product Description: | Goat Polyclonal anti-ALMS 1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide with the sequence RVTNQLLGRKVPWD representing the C-Terminus of the human protein (residues 4145-4167) according to NP_055935. |
| Specificity: | The C terminus of ALMS 1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. |
| CrossReactivity: | Expected to cross-react with Human and Mouse due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 200kDa and 75kDa in lysates of Human T cell line Jurkat after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cells. Recommended concentration, 3µg/ml. |
| Background: | This protein contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 195 |
| AppSummary: | ELISA, WB, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | anti-ALSS antibody, anti-Alstrom syndrome 1 antibody, anti-KIAA0328 antibody |
| ProteinTarget: | ALMS 1 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Collin GB et al. Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory degeneration in Alstram syndrome. Nat Genet 31:74-8 (2002). |
| NotesMain: | LocusLink ID - 7840 (human); 236266 (mouse). **Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Uses & Dilutions below). However no additional claims are made for their ability to recognize native protein in any application. |
| Gene_Id: | 7840 |
| Omim_ID: | H00000239-A01 |