| CatalogCode: | NB100-1161 |
| ProductName: | KAP 1 Antibody |
| Product Description: | Goat Polyclonal anti-KAP 1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: SSQELSGGPGDGP, representing the C-Terminus of the human protein (residues 741-753), according to NP_005753. |
| CrossReactivity: | Cross-reacts with Human and Mouse (see review). Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for Western Blot - use at an assay dependent dilution. Predicted molecular weight: 88.5 kDa. Not tested in other applications.Recommended Starting Dilution:*WB: 0.2-1ug/ml *Optimal dilutions/concentrations should be determined by the end user. |
| Control: | HepG2 whole cell lysate. |
| Background: | The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1). KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-KAP1 antibody, anti-KAP-1 antibody, anti-KRAB-associated Protein 1 antibody, anti-TRIM28 antibody, anti-Tripartite Motif-containing 28 antibody, anti-TF1B antibody, anti-TIF1B antibody, anti-Transcriptional Intermediary Factor 1-beta antibody, anti-RNF96 antibody |
| ProteinTarget: | KAP 1 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Friedman JR et al. KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev 10:2067-78 (1996). |
| Gene_Id: | 10155 |
| Omim_ID: | NB120-3472 |
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