| CatalogCode: | NB100-113 |
| ProductName: | PARP Antibody |
| Product Description: | Rabbit Polyclonal anti-PARP |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide based on sequence from C-terminal part of the bovine PARP automodification domain (residues 509-524), conjugated to a carrier. |
| Specificity: | Recognizes 116 kDa PARP and the 85 kDa apoptosis-related cleavage fragment. |
| CrossReactivity: | This antibody cross-reacts with human, bovine, rat, and mouse PARP. |
| Packaging: | 0.1 ml Whole antisera Rabbit antisera. |
| Uses: | This antibody can be used for Western blot and ELISA. |
| Background: | Poly(ADP-ribose) polymerase (PARP), a nuclear enzyme often termed a molecular nick sensor, recognizes and binds to DNA single or double strand breaks through the N-terminal DNA binding domain sensor. The activation of PARP is one of the first responses to DNA strand interruptions. The DNA-bound, catalytically activated PARP synthesizes poly(ADP-ribose)(pADPr) on nuclear target proteins such as topoisomerase, histones, and PARP itself. Although the exact role of PARP is not well known, it has been implicated in different cellular responses to genotoxic damage. These responses include cell survival and death, transformation, and DNA repair. Additional studies have demonstrated the role of PARP in the death of neuronal cells after ischemia-reperfusion injury, as well as maintaining the genomic integrity and survival responses of PARP knockout mice after exposure to whole body g-irradiation. |
| ProductRef: | 1. Lamarre, D., et al. Structural and funtional analysis of poly(ADP ribose) polymerase: an immunological study. Bioch. Biophys. Acta. 950: 147-160, 1988. 2. Lazebnik, Y.A., et al. Cleavage of poly(ADP ribose) polymerase by a poteinase with properties like ICE. Nature (Lond.). 371:346-347, 1994. 3. Datta, R., et al. Activation of a CrmA-insensitive, p35-sensitive Pathway in Ionizing Radiation-induced Apoptosis J. Biol. Chem. 272: 1965-1969, 1997. (Western blot, human) 4. Rhun, Y.L., et al, Cellular responses to DNA damage in the absence of poly(ADP-ribose)polymerase. Biochem. and Biophys. Res. Comm. 245:1-10, 1998. (mouse) |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Host_Name: | Rabbit |
| Buffer: | 0.2% Gelatin, PBS |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Bv, Hu, Mu, Rt |
| ALTnames: | anti-ADP ribosyltransferase NAD+ antibody, anti-pADPRT1 antibody, anti-PARP1 antibody, anti-Poly adenosine diphosphate ADP ribose polymerase antibody, anti-Poly ADP ribose polymerase 1 antibody, anti-Poly ADP ribose synthetase 1 antibody, anti-PPOL antibody |
| ProteinTarget: | PARP |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Duriez, P.J., et al. Biochem. Biophys. Acta. 1997, 1334, 65 |
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