| CatalogCode: | NB100-112 |
| ProductName: | PARP Antibody |
| Product Description: | Mouse Monoclonal anti-PARP (C-2-10) |
| Clone: | C-2-10 |
| Clonality: | Monoclonal |
| Immunogen: | Purified calf thymus poly(ADP-ribose) polymerase (PARP). |
| Epitope: | The C-terminal part of the DNA binding domain of PARP. |
| Specificity: | Recognizes the 116 kDa PARP and the 85 kDa apoptosis-related cleavage fragment. |
| CrossReactivity: | This antibody reacts with human, monkey, hamster, rat, and mouse. It does not react with chicken. |
| Packaging: | 0.05 ml Mouse ascites. |
| Uses: | NB 100-112 can be used in Western blot procedures, immunocytochemistry and ELISA. |
| Background: | Poly(ADP-ribose) polymerase (PARP), a nuclear enzyme often termed a molecular nick sensor, recognizes and binds to DNA single or double strand breaks through the N-terminal DNA binding domain sensor. The activation of PARP is one of the first responses to DNA strand interruptions. The DNA-bound, catalytically activated PARP synthesizes poly(ADP-ribse)(pADPr) on nuclear target proteins such as topoisomerase, histones, and PARP itself. Although the exact role of PARP is not well known, it has been implicated in different cellular responses to genotoxic damage. These responses include cell survival and death, transformation, and DNA repair. Additional studies have demonstrated the role of PARP in the death of neuronal cells after ischemia-reperfusion injury, as well as maintaining the genomic integrity and survival responses of PARP knockout mice after exposure to whole body gamma-irradiation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS, pH 7.2 |
| ListPrice: | 295 |
| NovusRef: | 1. Lo, P., et al. The Prosurvival Activity of p53 Protects Cells from UV-Induced Apoptosis by Inhibiting c-Jun NH(2)-terminal Kinase Activity and Mitochondrial Death Signaling. Cancer. 64: 8736-8745, (2004). (Western blot, Human) |
| AppSummary: | ELISA, WB, ICC |
| SpeciesSummary: | Ch(-), Ha, Hu, Mu, Mk, Rt |
| ALTnames: | anti-ADP ribosyltransferase NAD+ antibody, anti-pADPRT1 antibody, anti-PARP1 antibody, anti-Poly adenosine diphosphate ADP ribose polymerase antibody, anti-Poly ADP ribose polymerase 1 antibody, anti-Poly ADP ribose synthetase 1 antibody, anti-PPOL antibody |
| ProteinTarget: | PARP |
| PackageSize: | 0.05 ml |
| GeneralRef: | 1. Lamarre, D., et al. Structural and funtional analysis of poly(ADP ribose) polymerase: an immunological study. Bioch. Biophys. Acta. 950: 147-160, (1988). 2. Lazebnik, Y.A., et al. Cleavage of poly(ADP ribose) polymerase by a poteinase with properties like ICE. Nature (Lond.). 371: 346-347, (1994). 3. Datta, R., et al. Activation of a CrmA-insensitive, p35-sensitive Pathway in Ionizing Radiation-induced Apoptosis J. Biol. Chem. 272: 1965-1969, (1997). (Western blot, Human) 4. Rhun, Y.L., et al., Cellular responses to DNA damage in the absence of poly(ADP-ribose)polymerase. Biochem. and Biophys. Res. Comm. 245: 1-10, (1998). (mouse) |
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