| CatalogCode: | NB100-1110 |
| ProductName: | DCTN1 Antibody |
| Product Description: | Goat Polyclonal anti-DCTN1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: QEQLHQLHSRLIS, representing the C-Terminus of the human protein (residues 1266-1278), according to NP_004073; NP_075408. |
| Specificity: | This antibody is expected to recognise both human isoforms. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen) and Drosophilia melanogaster (90% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for ELISA and Western Blot. In Western Blot, approx 150kDa band observed in A549 and Human Testis lysates. (predicted MW of 147kDa).Not tested in other applications.Recommended Starting Dilution:*ELISA: 1/28000WB: 0.5 - 2 ug/ml*Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | A549 and Human Testis lysates. |
| Background: | The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-150 kDa dynein-associated polypeptide antibody, anti-dynactin 1 (p150 glued homolog Drosophila) antibody, anti-Dynactin 1 antibody |
| ProteinTarget: | DCTN1 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Holzbaur EL & Tokito MK Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization. Genomics 31:398-9 (1996). |
| Gene_Id: | 1639 |
| Omim_ID: | H00010540-M01 |