| CatalogCode: | NB100-1085 |
| ProductName: | GATA 3 Antibody |
| Product Description: | Goat Polyclonal anti-GATA 3 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide EVTADQPRWVSHH, representing the N Terminus of the human protein (residues 2-14), according to NP_002042. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for Western Blot and Immunohistrochemistry. Approx 45-50kDa band seen in MOLT-4 lysate in western analysis. In paraffin-embedded human tonsil, stains nuclei of some cells in the interfollicular area and in germinal centers.
Not tested in other applications.Recommended Starting Dilution:*WB: Recommended for use at 2.0 - 4.0ug/mlIHC-P: Recommended for use at 0.1 - 0.3ug/ml*Optimal working dilutions / concentrations should be determined by the end user. |
| Control: | MOLT 4 lysate. |
| Background: | The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells, macrophages, or HeLa cell lines. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | IHC-P, WB, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | anti-GATA-binding protein 3 antibody, anti-HDR antibody, anti-MGC5445 antibody |
| ProteinTarget: | GATA 3 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Marine J & Winoto A The human enhancer-binding protein Gata3 binds to several T-cell receptor regulatory elements. Proc Natl Acad Sci U S A 88:7284-8 (1991). |
| Gene_Id: | 2625 |
| Omim_ID: | NB100-78303 |