| CatalogCode: | NB100-106SS |
| ProductName: | Ogg1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Ogg1 |
| Clonality: | Polyclonal |
| Immunogen: | A peptide derived from the human Ogg1 (within amino acids 1-100). |
| Specificity: | This antibody is specific for hOgg1. |
| CrossReactivity: | Human, primate, and rat. Other species have not been tested. |
| Packaging: | 0.025 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody may be used in Western blot where it recognizes a band at ~39 kDa, representing Ogg1 (tested in human). For immunohistochemistry it has been tested on human and primate. It may also work on rat in immunohistochemistry (see Novus Specific reference 5 - Dobson, AW, et al). |
| Localization: | Mitochondrial and Nuclear |
| Control: | Jurkat whole cell extracts (NB 800-PC2) |
| Background: | 8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. hOgg1 proteins efficiently release the 8-hydroxyguanine opposite the pyrimidine from DNA and cleave the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The hOgg1 gene has been mapped to region 3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer. Recent work has demonstrated that Ogg plays an important role in CAG expansion, a characteristic of several neurodegenerative diseases. Ogg appears to be responsible for progressive expansion of poly-Q tracts in response to oxidative damage. Thus, Ogg provides a direct link between DNA damage and toxicity in neurons. |
| ProductRef: | 1. Chen, D., et al. The p53 Pathway Promotes Efficient Mitochondrial DNA Base Excision Repair in Colorectal Cancer Cells. Cancer Res. 66: 3485-3494. (2006) 2. Rachek, L. I., et al. Protection of INS-1 Cells From Free Fatty Acid-Induced Apoptosis by Targeting hOGG1 to Mitochondria. Diabetes. 55: 1022-1028. (2006) 3. Roseborough, G., et al. The Mitochondrial K-ATP Channel Opener, Diazoxide, Prevents Ischemia-Reperfusion Injury in the Rabbit Spinal Cord. Amer. J. Pathol. 168: 1443-1451. (2006) 4. Xydas, S., et al. beta2-Adrenergic Stimulation Attenuates Left Ventricular Remodeling, Decreases Apoptosis, and Improves Calcium Homeostasis in a Rodent Model of Ischemic Cardiomyopathy. J. Pharmacol. and Exper. Therap. 317: 553-561. (2006) |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 100 |
| NovusRef: | 1. Lin, L., et al. Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion. J. Neurochemistry. 74: 1098-1105. (2000) 2. Mambo, E., et al. Defective Repair of 8-Hydroxyguanine in Mitochondria of MCF-7 and MDA-MB-468 Human Breast Cancer Cell Lines. Cancer Res. 62: 1349-1355. (2002) 3. Fan, C., et al. Frequent Allelic Imbalance and Loss of Protein Expression of the DNA Repair Gene hOGG1 in Head and Neck Squamous Cell Carcinoma. Lab. Invest. 81: 1429-1438. (2001) 4. Tyrberg, B., et al. Islet expression of the DNA repair enzyme 8-oxoguanosine DNA glycosylase (Ogg1) in human type 2 diabetes. Biomed Central: www.biomedcentral.com/1472-6823/2/2prepub 5. Dobson, A.W., et al. Enhanced mtDNA repair capacity protects pulmonary artery endothelial cells from oxidant-mediated death. Am J Physiol Lung Cell Mol Physiol. 283: L205-L210. (2002) 6. Tuo. J., et al. Functional crosstalk betweew hOgg1 and the helicase domain of Cokayne syndrome group B protein. DNA Repair. 1:931-927. (2002) 7. Potts, R., et al. Cadmium exposure down-regulates 8-oxoguanine DNA glyosylase expression in rat lung and alolar epithelial cells. Toxicology. 184: 189-202. (2003) 8. Rachek, L., et al. Conditional Targeting of the DNA Repair Enzyme hOGG1 into Mitochondria. JBC. 277(47): 44932-44937. (2002) 9. Ruchko, M., et al. Mitochondrial DNA Damage Triggers Mitochondrial Dysfunction and Apoptosis in Oxidant-challenged Lung Endothelial Cells. Am J Physiol Lung Cell Mol Physiol. 10.1152/ajplung.00255.2004. 10. Buckley, S., et al. In vivo inosine protects alveolar epithelial type 2 cells against hyperoxia-induced DNA damage through MAP Kinase signaling. Am J Physiol Lung Cell Mol Physiol. 10.1152/ajplung.00278.2004. 11. Stuart, J., et al. DNA base excision repair activities and pathway function in mitochondrial and cellular lysates from cells lacking mitochondrial DNA. Nucleic Acids Res. 32: 2181-2192. (2004) 12. Hashiguchi, K., et al. The C-terminal alpha O helix of human Ogg1 is essential fro 8-oxoguanine DNA glycosylase activity: the mitochondrial beta-Ogg1 lacks this domain and does not have glycosylase activity. Nucleic Acids Res. 32(18): 5596-5608. (2004) 13. Druzhyna, N.M. et al. Cytokines induce NO-mediated mtDNA damage and apoptosis in oligodendrocytes. Protective role of targeting 8-oxoguanine glycosylase to mitochondria. JBC. 280(22): 21673-21679. (2005) [Western blot, Rat] 14. Imai, K., et al. Induction of OGG1 gene expression by HIV-1 tat. JBC. 280(29): 26701-26713. (2005) [Western blot, Human] 15. Ashok, B.T. & Ali, R. Binding of circulating antibodies to reactive oxygen species modified-DNA and detecting DNA damage by a monoclonal antibody probe. Mechan. Aging and Devel. 103:69-80. (1998) [Bovine] 16. Ashok, B.T., et al. Anti-Ros-DNA monoclonal antibody as molecular probe for oxidative DNA damage. Biochem. & Mol. Bio. Int. 43(6): 1219-1229. (1997) [Bovine] 17. Radicella, J.P., et al. Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae. Genetics. 94:8010-8015. (1997) [Bacteria, Yeast] 18. Monden, Y., et al. Human MMH (OGG!) type 1a protien is a major enzyme for repair of 8-hydroxyguanine lesions in human cells. Biochem. & Biophys. Res. Comm. 258:605-610. (1999) [Western Blot, Immunoprecipitation, Human] 19. Sakumi, K. et al. Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-Oxo-dGTP, a mutagenic substrate for DNA synthesis. JBC. 268(31): 23524-23530. (1993) [Human] 20. Lu, R. et al. A mammilian DNA repair enzyme that excises oxidatively damaged guanines maps to a locus frequently lost in lung cancer. Curr. Bio. 7: 397-407. (1997) [Mouse, Human] 21. Wikman, H. et al. hOGG1 plymorphism and loss of heterogosity (LOH): significance for lung cancer susceptibility in a caucasian population. Int. J. of Cancer 88: 932-937. (2000) [Human] 22. Tsurudome, Y., et al. Changes in levels of 8-hydroxyguanine in DNA, its repair and OGG1 mRNA in rat lungs after intratracheal administraion of diesel exhaust particles. Carcinogenesis. 20(8): 1573-1576. (1999) [Rat] 23. Hilbert, T.P., et al. Cloning and expression of the cDNA encoding the human homologue of the DNA repair enzyme, echerichia coli endonuclease III. JBC. 272(10): 6733-6740. (1997) [Northen Blot, Human] 24. Chatterjee, A., et al. The effect of p53-RNAi and p53 knockout on human 8-oxoguanine DNA glycosylase (hOgg1) activity. FASEB J. (2005) 25. Xydas, S., et al. beta2-adrenergic stimulation attenuates left ventricular remodeling, decreases apoptosis, and improves calcium homeostasis in a rodent model of ischemic cardiomyopathy. J. Pharmacol. Exp. Ther. 317: 553-561. (2006) 26. Rachek, L.I., et al. Protection of INS-1 cells from free fatty acid-induced apoptosis by targeting hOGG1 to mitochondria. Diabetes. 55: 1022-1028. (2006) 27. Chen, D., et al. The p53 pathway promotes efficient mitochondrial DNA base excision repair in colorectal cancer cells. Cancer Res. 66: 3485-3494. (2006) |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Hu, Mk, Rt |
| ALTnames: | Anti-8-oxoguanine DNA glycosylase antibody; Anti-DNA lyase antibody; Anti-DNA-(apurinic or apyrimidinic site) lyase antibody; Anti-HMMH antibody; Anti-HOGG 1 antibody; Anti-HOGG1 antibody; Anti-MUTM antibody; Anti-N-glycosylase antibody; Anti-Ogg 1 antibody; Anti-OGH 1 antibody; Anti-OGH1 antibody |
| PackageSize: | 0.025 ml |
| Homology: | Rat - 80% identity/homology Mouse - 86% identity/homology |
| NotesMain: | Production was supported by SBIR grant R43CA83507-01. |
| Gene_Symbol: | OGG1 |
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