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| Catalog Code: | BM4008F |
| Product Name: | F4/80 Antigen - FITC Antibody |
| Product Description: | Rat Monoclonal anti-F4/80 Antigen - FITC (A3-1 (F4/80)) |
| Clone: | A3-1 (F4/80) |
| Clonality: | Monoclonal |
| ListPrice: | 515 |
| Concentration: | 0.1 mg/ml |
| Immunogen: | Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice. |
| Specificity: | This antibody recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (5). |
| Packaging: | 0.1 mg This antibody is supplied as 0.1 mg liquid, Protein G affinity purified Ig fraction conjugated to Fluorescein Isothiocyanate Isomer I (FITC), iPhosphate buffered saline pH7.4, containing 0.09% Sodium Azide (NaN3)as preservative and 1% BSA as stabilizer. |
| Uses: | Flow cytometry: use 10 ul of neat antibody to label 106 cells in 100 ul. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Storage: | Store at 4C. Do not freeze. |
| Isotype: | IgG2b |
| Host Name: | Rat |
| Application Summary: | flow cytometry / FACS analysis |
| Package Size: | 0.1 mg |
| Notes Main: | This product is photosensitive and should be protected from light. |
| General References: | 1. Austyn, J.M. and Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815. 2. Hume, D.A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80: Macrophages associated with epithelia. The Anatomical Record 210: 503-512. 3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80. J. Exp. Med. 161: 475-489. 4. Gordon, S. et al. (1992) Antigen markers of macrophage differentitation in murine tissues. Curr. Top. Microbiol. Immunol. 181: 1-37. 5. Warschkau, H. and Kiderlen, A.F. (1999) A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes. J. Immunol.163:3409 |
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